EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.1.3.13 | -999 |
- |
in a commercial preparation, one of AOX's isozymes is more resistant to thermal inactivation at atmospheric or high pressures. The resistant fraction is 4-34 times more stable than the labile fraction. High hydrostatic pressure stabilizes AOX against thermal inactivation. A 14fold stabilization is observed at 49.4°C for the labile fraction at 200 MPa relative to atmospheric pressure. The activation energy of inactivation at 40-160 MPa ranges from 95 to 184 kJ per mol for the resistant fraction and from 232 to 402 kJ per mol for the labile fraction |
763331 |
| 1.1.3.13 | -999 |
- |
presence of alginic acid (0.35%) enhances thermal stability, resulting in 72% increase in its half-life at 40°C under the operational conditions |
763667 |
| 1.1.3.13 | -999 |
- |
there are two fractions of AOx with different thermal stability. High hydrostatic pressure increases the activity of the heat labile (L) + resistant (R) combined fractions but not of the R fraction alone. The activity of the L + R fractions increases 2.4fold at 160 MPa and 30°C compared to the activity at 0.1 MPa. At higher temperatures, the increase in activity with pressure is greater due to the combined stabilization and activation effects. The reaction rate of the R fraction at 50 °C is 17.9 or 17.7 microM min-1 at 80 or 160 MPa, respectively, and is not significantly different from the activity of the L + R fractions under the same conditions (18.4 micro min-1) |
762989 |
| 1.1.3.13 | 4 |
- |
1 month, more than 50% of the activity is retained |
685404 |
| 1.1.3.13 | 4 |
- |
24 h, loss of only 5% of activity |
671539 |
| 1.1.3.13 | 20 |
- |
24 h stable |
484914 |
| 1.1.3.13 | 20 |
60 |
10% loss of activity at 20°C after 24 h, complete loss of activity after 10 min at 60°C |
711177 |
| 1.1.3.13 | 22 |
- |
50% loss of activity after 10 min in 0.1 M potassium phosphate buffer, pH 6.0. 50% loss of activity after 60 min in 0.1 M potassium phosphate buffer, pH 7.0, 50% loss of activity after 28 h in 0.1 M Tris-HCl buffer, pH 8.0. 50% loss of activity after 16 h in 0.1 M Tris-HCl buffer, pH 9.0 |
484922 |
| 1.1.3.13 | 25 |
- |
pH 5.0, 3 h, complete inactivation at pH 5.0 after 3 h |
484905 |
| 1.1.3.13 | 30 |
- |
10 min, stable |
484907 |
