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Literature summary for 1.1.3.13 extracted from
- Purification and properties of a novel broad substrate specific alcohol oxidase from Aspergillus terreus MTCC 6324 (2008), Biochim. Biophys. Acta, 1784, 1552-1559.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ferrocene | 1 mM, increases the alcohol oxidase activity only for the short chain alcohol substrate to 47% of the original activity | Aspergillus terreus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,12-dichlorododecane | - |
Aspergillus terreus | |
| 2-mercaptoethanol | - |
Aspergillus terreus | |
| AgNO3 | 1 mM, complete loss of activity | Aspergillus terreus | |
| PCMB | 5 mM, complete loss of activity | Aspergillus terreus | |
| Tetraethylthiuram disulfide | 5 mM, more than 70% loss of activity | Aspergillus terreus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.498 | - |
n-Heptanol | - |
Aspergillus terreus | |
| 1.86 | - |
n-dodecanol | - |
Aspergillus terreus | |
| 2.3 | - |
phenyl-3-propanol | - |
Aspergillus terreus | |
| 18.3 | - |
(R)-2-octanol | - |
Aspergillus terreus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Aspergillus terreus | 16020 | - |
| microsome | - |
Aspergillus terreus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| MgCl2 | 1 mM, increases the enzyme activity to nearly 50% for both short chain-, and long chain alcohol substrates | Aspergillus terreus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
| 27000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
| 43000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
| 63000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
| 85000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
| 269000 | - |
non-denaturing PAGE | Aspergillus terreus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus terreus | - |
MTCC 6324 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| lipoprotein | presence of oleic acid and palmitic acid at a ratio of 2:1 in the purified protein. This lipoidic nature of the protein particles is correlated to the high aggregating property | Aspergillus terreus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus terreus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:n-hexadecane grown-cell | - |
Aspergillus terreus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 1 month, more than 50% of the activity is retained | Aspergillus terreus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-octanol + O2 | - |
Aspergillus terreus | 2-octanone + H2O2 | - |
? | |
| n-dodecanol + O2 | - |
Aspergillus terreus | n-dodecanal + H2O2 | - |
? | |
| n-heptanol + O2 | substrates with highest affinity | Aspergillus terreus | n-heptanal + H2O2 | - |
? | |
| phenyl-3-propanol + O2 | - |
Aspergillus terreus | 3-phenylpropanal | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| broad substrate specific alcohol oxidase | - |
Aspergillus terreus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
1 month, more than 50% of the activity is retained | Aspergillus terreus |
| 32 | - |
2 h, 80% loss of activity | Aspergillus terreus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 270 | - |
n-Heptanol | - |
Aspergillus terreus | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
3 h, 5% loss of activity | Aspergillus terreus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavin is non-covalently but avidly associated. Peptide mass fingerprinting studies show the presence of two FAD binding domains in 63 kDa protein | Aspergillus terreus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000014 | - |
2-mercaptoethanol | substrate: R-(-)-2-octanol | Aspergillus terreus | |
| 0.00063 | - |
2-mercaptoethanol | substrate: n-dodecanol | Aspergillus terreus | |
| 0.00157 | - |
2-mercaptoethanol | substrate: n-heptanol | Aspergillus terreus | |
| 0.00246 | - |
1,12-dichlorododecane | substrate: n-dodecanal | Aspergillus terreus | |
| 0.00362 | - |
2-mercaptoethanol | substrate: phenyl-3-propanol | Aspergillus terreus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus terreus | isoelectric focusing, pH-range: 3-10 | 8.5 | 8.3 |
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