2.7.1.63: polyphosphate-glucose phosphotransferase
This is an abbreviated version!
For detailed information about polyphosphate-glucose phosphotransferase, go to the full flat file.
Word Map on EC 2.7.1.63
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2.7.1.63
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mycobacterium
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tuberculosis
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6-phosphate
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glucokinases
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shermanii
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propionibacterium
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nonprocessive
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synthesis
- 2.7.1.63
- mycobacterium
- tuberculosis
- 6-phosphate
- glucokinases
- shermanii
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propionibacterium
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nonprocessive
- synthesis
Reaction
Synonyms
All1371, GMK, inorganic polyphosphate/ATP-glucomannokinase, inorganic polyphosphate:D-glucose 6-phosphotransferase, More, PGPTase, phosphotransferase, polyphosphate-glucose, poly P glucokinase, poly(P) glucokinase, poly(P)/ATP-glucomannokinase, PolyP-dependent glucokinase, polyP-GK, polyP-Glk, polyphosphate glucokinase, polyphosphate-D-(+)-glucose-6-phosphotransferase, polyphosphate-dependent glucokinase, polyphosphate-glucose 6-phosphotransferase, polyphosphate/ATP-glucomannokinase, PP-Glk, PPGK, SCO5059
ECTree
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Crystallization
Crystallization on EC 2.7.1.63 - polyphosphate-glucose phosphotransferase
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purified recombinant enzyme, hanging drop vapour diffusion method, cyrstallization drop on a silconized coverslip by mixing of 0.003 ml protein solution consisting of 10 mg/ml protein in 10 mM glucose, and 10 mM potassium phosphate, pH 7.0, with an equal volume of mother liquor composed of 2.0 M ammonium sulfate, 2% v/v PEG 400, and 0.1 M HEPES, pH 8.0, cyrstals are soaked in several heavy atom derivative solutions containing 1 mM of UO2Ac2, 10 mM AgNO3, 10 mM GdCl2, 1 mM HgCl2, 10 mM K2Pt(CN)4, 2 mM TmCl2, or 1 mM EuCl3 in 0.1 M Tris-HCl at different pH-values, dependent on the heavy atom salt, for 20-60 min at 20°C, X-ray diffraction structure determiination and analysis at 3.0 A resolution, modeling
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