loss-of-function for Ins(1,3,4)P3 in the H162D mutant is matched by a 200fold reduction in its Ins(1,3,4)P3 kinase activity. The H162D mutant dephosphorylats Ins(1,3,4,5,6)P5 almost twice as fast as the wild-type enzyme. The H162D mutant also displays very limited ability to transfer phosphate from Ins(1,3,4,5,6)P5 to Ins(1,3,4)P3 in the presence of physiological concentrations of nucleotide
ositpk6 mutant, the line with the P522L amino acid substitution has agronomic performance (seed weight, germination, and seedling growth) similar to that of its wild-type parent
generation of itpk1-1 mutants, phenotype, overview. Genetic interaction analysis of bifunctional ITPK1 (EC 2.7.1.159/EC 2.7.1.134) and IPK1 (EC 2.7.1.158) with a genetic cross between ipk1-1 and itpk1 mutants. The ipk1-1 itpk1 double mutants exhibit more severe growth defects than single mutants and those that proceed to the reproductive stage bore aborted seeds. Common elevation of D/L-Ins(3,4,5,6)P4 in itpk1 and ipk1-1 mutants
generation of itpk1-1 mutants, phenotype, overview. Genetic interaction analysis of bifunctional ITPK1 (EC 2.7.1.159/EC 2.7.1.134) and IPK1 (EC 2.7.1.158) with a genetic cross between ipk1-1 and itpk1 mutants. The ipk1-1 itpk1 double mutants exhibit more severe growth defects than single mutants and those that proceed to the reproductive stage bore aborted seeds. Common elevation of D/L-Ins(3,4,5,6)P4 in itpk1 and ipk1-1 mutants
OsITPK6 mutants are generated by CRISPR/Cas9-mediated mutagenesis, generation of 23 hygromycin phosphotransferase (HPT)-positive T0 plants transformed with the CRISPR/Cas9 vector pH-itpk6. Genotyping of gene ITPK6, the ositpk6_1 (a 6-nt in-frame deletion) mutation results in the loss of two amino acids at positions 91 to 92. In contrast, all the other three mutations, i.e. ositpk6_2 (a 1-nt insertion), ositpk6_3 (a 5-nt deletion), and ositpk6_4 (a 2-nt deletion), generate a premature stop codon almost right after the mutation site and, hence, significantly shorten the encoded proteins. Consequently, the ositpk6_2, _3 and _4 mutant alleles are predicted to produce proteins of only 105, 103, and 104 amino acids, respectively. The two amino acids missing in the ositpk6_1 mutant are located in a highly conserved segment, suggesting the mutation of ositpk6_1 might have a potential functional consequence. Plant growth of mutants ositpk6_2, _3, and _4 is significantly impaired. Their panicles are significantly shorter (30.1%, 28.8%, and 29.1%, respectively) compared to wild-type parental cultivar Xidao 1, phenotypes, overview. Numbers and lengths of root from mutant plants are reduced under salt stress compared to wild-type