2.6.1.82: putrescine-2-oxoglutarate transaminase
This is an abbreviated version!
For detailed information about putrescine-2-oxoglutarate transaminase, go to the full flat file.
Word Map on EC 2.6.1.82
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2.6.1.82
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cadaverine
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synthesis
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l-lysine
-
gamma-aminobutyric
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gamma-aminobutyraldehyde
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agmatine
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transamination
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glutamicum
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volumetric
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corynebacterium
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5-aminovalerate
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pyrroline
-
putida
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adaptor
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aminotransferases
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synchrotron
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l-arginine
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clpap
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n-degron
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catabolite
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klebsiella
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l-ornithine
-
polyamide
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semialdehyde
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diamines
- 2.6.1.82
- cadaverine
- synthesis
- l-lysine
-
gamma-aminobutyric
- gamma-aminobutyraldehyde
- agmatine
-
transamination
- glutamicum
-
volumetric
-
corynebacterium
- 5-aminovalerate
-
pyrroline
- putida
- adaptor
- aminotransferases
-
synchrotron
- l-arginine
- clpap
-
n-degron
-
catabolite
-
klebsiella
- l-ornithine
- polyamide
- semialdehyde
-
diamines
Reaction
Synonyms
FG99_07980, KES24511, PatA, PATase, Pp-SpuC, putrescine aminotransferase, putrescine transaminase, putrescine-alpha-ketoglutarate transaminase, putrescine:2-oxoglutarate aminotransferase, SpuC, YgjG, YjgG
ECTree
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Substrates Products
Substrates Products on EC 2.6.1.82 - putrescine-2-oxoglutarate transaminase
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REACTION DIAGRAM
1H-indole-2-carbaldehyde + isopropylamine
1-(1H-indol-2-yl)methanamine + acetone + H2O
-
-
-
?
2,3-dimethoxybenzaldehyde + cadaverine
3,5-dimethoxybenzylamine + piperideine + H2O
-
-
-
?
2,3-dimethoxybenzaldehyde + isopropylamine
2,3-dimethoxybenzylamine + acetone + H2O
-
-
-
?
2,4-dimethoxybenzaldehyde + cadaverine
2,4-dimethoxybenzylamine + piperideine + H2O
-
-
-
?
2,4-dimethoxybenzaldehyde + isopropylamine
2,4-dimethoxybenzylamine + acetone + H2O
-
-
-
?
2-(trifluoromethyl)benzaldehyde + cadaverine
2-(trifluoromethyl)benzylamine + piperideine + H2O
-
-
-
?
2-(trifluoromethyl)benzaldehyde + isopropylamine
2-(trifluoromethyl)benzylamine + acetone + H2O
-
-
-
?
2-formylbenzoic acid + cadaverine
2-(aminomethyl)benzoic acid + piperideine + H2O
-
-
-
?
2-formylbenzoic acid + isopropylamine
2-(aminomethyl)benzoic acid + acetone + H2O
-
-
-
?
2-formylbenzonitrile + cadaverine
2-(aminomethyl)benzonitrile + piperideine + H2O
-
-
-
?
2-formylbenzonitrile + isopropylamine
2-(aminomethyl)benzonitrile + acetone + H2O
-
-
-
?
2-methoxybenzaldehyde + cadaverine
2-methoxybenzylamine + piperideine + H2O
-
-
-
?
2-methoxybenzaldehyde + isopropylamine
2-methoxybenzylamine + acetone + H2O
-
-
-
?
2-methoxypyridine-3-carbaldehyde + cadaverine
1-(2-methoxypyridin-3-yl)methanamine + piperideine + H2O
-
-
-
?
2-methoxypyridine-3-carbaldehyde + isopropylamine
1-(2-methoxypyridin-3-yl)methanamine + acetone + H2O
-
-
-
?
2H-1,3-benzodioxole-4-carbaldehyde + cadaverine
1-(2H-1,3-benzodioxol-4-yl)methanamine + piperideine + H2O
-
-
-
?
2H-1,3-benzodioxole-4-carbaldehyde + isopropylamine
1-(2H-1,3-benzodioxol-4-yl)methanamine + acetone + H2O
-
-
-
?
3,4-difluorobenzaldehyde + cadaverine
3,4-difluorobenzylamine + piperideine + H2O
-
-
-
?
3,4-difluorobenzaldehyde + isopropylamine
3,4-difluorobenzylamine + acetone + H2O
-
-
-
?
3,5-dibromobenzaldehyde + cadaverine
3,5-dibromobenzylamine + piperideine + H2O
-
-
-
?
3,5-dibromobenzaldehyde + isopropylamine
3,5-dibromobenzylamine + acetone + H2O
-
-
-
?
3,5-dimethoxybenzaldehyde + isopropylamine
3,5-dimethoxybenzylamine + acetone + H2O
-
-
-
?
3-acetylbenzoic acid + cadaverine
3-(1-aminoethyl)benzoic acid + piperideine + H2O
-
-
-
?
3-acetylbenzoic acid + isopropylamine
3-(1-aminoethyl)benzoic acid + acetone + H2O
-
-
-
?
3-chlorobenzaldehyde + cadaverine
3-chlorobenzylamine + piperideine + H2O
-
-
-
?
3-chlorobenzaldehyde + isopropylamine
3-chlorobenzylamine + acetone + H2O
-
-
-
?
3-fluorobenzaldehyde + cadaverine
3-fluorobenzylamine + piperideine + H2O
-
-
-
?
3-fluorobenzaldehyde + isopropylamine
3-fluorobenzylamine + acetone + H2O
-
-
-
?
3-formylbenzonitrile + cadaverine
3-(aminomethyl)benzonitrile + piperideine + H2O
-
-
-
?
3-formylbenzonitrile + isopropylamine
3-(aminomethyl)benzonitrile + acetone + H2O
-
-
-
?
3-methoxybenzaldehyde + cadaverine
3-methoxybenzylamine + piperideine + H2O
-
-
-
?
3-methoxybenzaldehyde + isopropylamine
3-methoxybenzylamine + acetone + H2O
-
-
-
?
3-nitrobenzaldehyde + cadaverine
3-nitrobenzylamine + piperideine + H2O
-
-
-
?
3-nitrobenzaldehyde + isopropylamine
3-nitrobenzylamine + acetone + H2O
-
-
-
?
4-acetylbenzoic acid + cadaverine
4-(1-aminoethyl)benzoic acid + piperideine + H2O
-
-
-
?
4-acetylbenzoic acid + isopropylamine
4-(1-aminoethyl)benzoic acid + acetone + H2O
-
-
-
?
4-chlorobenzaldehyde + cadaverine
4-chlorobenzylamine + piperideine + H2O
-
-
-
?
4-chlorobenzaldehyde + isopropylamine
4-chlorobenzylamine + acetone + H2O
-
-
-
?
4-fluorobenzaldehyde + cadaverine
4-fluorobenzylamine + piperideine + H2O
-
-
-
?
4-fluorobenzaldehyde + isopropylamine
4-fluorobenzylamine + acetone + H2O
-
-
-
?
4-formylbenzoic acid + cadaverine
4-(aminomethyl)benzoic acid + piperideine + H2O
-
-
-
?
4-formylbenzoic acid + isopropylamine
4-(aminomethyl)benzoic acid + acetone + H2O
-
-
-
?
4-formylbenzonitrile + cadaverine
4-(aminomethyl)benzonitrile + piperideine + H2O
-
-
-
?
4-formylbenzonitrile + isopropylamine
4-(aminomethyl)benzonitrile + acetone + H2O
-
-
-
?
4-nitrobenzaldehyde + cadaverine
4-nitrobenzylamine + piperideine + H2O
-
-
-
?
4-nitrobenzaldehyde + isopropylamine
4-nitrobenzylamine + acetone + H2O
-
-
-
?
5-chlorothiophene-2-carbaldehyde + cadaverine
1-(5-chlorothiophen-2-yl)methanamine + piperideine + H2O
-
-
-
?
5-chlorothiophene-2-carbaldehyde + isopropylamine
1-(5-chlorothiophen-2-yl)methanamine + acetone + H2O
-
-
-
?
5-formylthiophene-2-carboxylic acid + cadaverine
5-(aminomethyl)thiophene-2-carboxylic acid + piperideine + H2O
-
-
-
?
5-formylthiophene-2-carboxylic acid + isopropylamine
5-(aminomethyl)thiophene-2-carboxylic acid + acetone + H2O
-
-
-
?
5-methylfuran-2-carbaldehyde + cadaverine
1-(5-methylfuran-2-yl)methanamine + piperideine + H2O
-
-
-
?
5-methylfuran-2-carbaldehyde + isopropylamine
1-(5-methylfuran-2-yl)methanamine + acetone + H2O
-
-
-
?
6-acetylpyridine-2-carboxylic acid + cadaverine
6-(1-aminoethyl)pyridine-2-carboxylic acid + piperideine + H2O
-
-
-
?
6-acetylpyridine-2-carboxylic acid + isopropylamine
6-(1-aminoethyl)pyridine-2-carboxylic acid + acetone + H2O
-
-
-
?
6-methoxypyridine-3-carbaldehyde + cadaverine
1-(6-methoxypyridin-3-yl)methanamine + piperideine + H2O
-
-
-
?
6-methoxypyridine-3-carbaldehyde + isopropylamine
1-(6-methoxypyridin-3-yl)methanamine + acetone + H2O
-
-
-
?
alpha-ketobutyric acid + putrescine
L-aspartate + 4-aminobutanal
38% activity
-
-
?
cadaverine + 2-oxoglutarate
L-glutamate + 5-aminopentanal
97% activity
-
-
?
cadaverine + 2-oxoglutarate
piperideine + L-glutamate + H2O
-
-
-
?
isopropylamine + 2-oxoglutarate
acetone + L-glutamate + H2O
-
-
-
?
quinoline-3-carbaldehyde + cadaverine
1-(quinolin-3-yl)methanamine + piperideine + H2O
-
-
-
?
quinoline-3-carbaldehyde + isopropylamine
1-(quinolin-3-yl)methanamine + acetone + H2O
-
-
-
?
1-(1H-indol-2-yl)methanamine + piperideine + H2O
-
-
-
?
1H-indole-2-carbaldehyde + cadaverine
1-(1H-indol-2-yl)methanamine + piperideine + H2O
Pseudomonas putida NBRC 14161
-
-
-
?
3,5-dimethoxybenzylamine + piperideine + H2O
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-
-
?
3,5-dimethoxybenzaldehyde + cadaverine
3,5-dimethoxybenzylamine + piperideine + H2O
Pseudomonas putida NBRC 14161
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-
-
?
1-pyrroline + L-glutamate + H2O
-
-
-
?
putrescine + 2-oxoglutarate
1-pyrroline + L-glutamate + H2O
overall reaction
-
-
?
putrescine + 2-oxoglutarate
1-pyrroline + L-glutamate + H2O
Pseudomonas putida NBRC 14161
overall reaction
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-
?
putrescine + 2-oxoglutarate
1-pyrroline + L-glutamate + H2O
-
overall reaction
-
-
?
putrescine + 2-oxoglutarate
1-pyrroline + L-glutamate + H2O
-
overall reaction
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-
?
putrescine + 2-oxoglutarate
L-glutamate + 4-aminobutanal
-
coupling action of Escherichia coli YgjG putrescine transaminase and YdcW dehydrogenase
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-
?
putrescine + 2-oxoglutarate
L-glutamate + 4-aminobutanal
putrescine best amino group donor for the enzyme, 100% activity
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-
?
?
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enzyme prefers diaminoalkanes as substrates and thereby generates cyclic imines from the omega-amino aldehyde intermediates
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-
?
additional information
?
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only modified PATase is a ClpS-dependent ClpAP substrate
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-
?
additional information
?
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enzyme prefers diaminoalkanes as substrates and thereby generates cyclic imines from the omega-amino aldehyde intermediates
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-
?
additional information
?
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enzyme prefers diaminoalkanes as substrates and thereby generates cyclic imines from the omega-amino aldehyde intermediates
-
-
?
additional information
?
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-
enzyme prefers diaminoalkanes as substrates and thereby generates cyclic imines from the omega-amino aldehyde intermediates
-
-
?
additional information
?
-
the enzyme shows dual substrate recognition for biogenic diamines (i.e. cadaverine) and readily available monoamines (i.e. isopropylamine) for the synthesis of benzylamine derivatives with excellent product conversions and extremely broad substrate tolerance. Biocatalytic synthesis of a range of substituted benzylamines mediated by Pp-SpuC in a coupled assay approach with cadaverine or isopropylamine as co-substrate at 50 mM Tris-HCl, pH 9.0, 30°C, 1 mM PLP, and 1% v/v DMSO, overview
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-
-
additional information
?
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-
the enzyme shows dual substrate recognition for biogenic diamines (i.e. cadaverine) and readily available monoamines (i.e. isopropylamine) for the synthesis of benzylamine derivatives with excellent product conversions and extremely broad substrate tolerance. Biocatalytic synthesis of a range of substituted benzylamines mediated by Pp-SpuC in a coupled assay approach with cadaverine or isopropylamine as co-substrate at 50 mM Tris-HCl, pH 9.0, 30°C, 1 mM PLP, and 1% v/v DMSO, overview
-
-
-
additional information
?
-
Pseudomonas putida NBRC 14161
the enzyme shows dual substrate recognition for biogenic diamines (i.e. cadaverine) and readily available monoamines (i.e. isopropylamine) for the synthesis of benzylamine derivatives with excellent product conversions and extremely broad substrate tolerance. Biocatalytic synthesis of a range of substituted benzylamines mediated by Pp-SpuC in a coupled assay approach with cadaverine or isopropylamine as co-substrate at 50 mM Tris-HCl, pH 9.0, 30°C, 1 mM PLP, and 1% v/v DMSO, overview
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-
-
additional information
?
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the activity of KES24511 is determined using an alanine dehydrogenase enzyme-coupled assay, the purified protein exhibited activity with two substrates, putrescine and 12-aminododecanoic acid, with pyruvate as the co-substrate for transamination. The transaminase reaction co-product (L-alanine) is converted in situ by the dehydrogenase in an NAD+-dependent oxidation, and nicotinamide conversion is spectrophotometrically monitored by the change in UV absorbance at 340 nm
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-
additional information
?
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the activity of KES24511 is determined using an alanine dehydrogenase enzyme-coupled assay, the purified protein exhibited activity with two substrates, putrescine and 12-aminododecanoic acid, with pyruvate as the co-substrate for transamination. The transaminase reaction co-product (L-alanine) is converted in situ by the dehydrogenase in an NAD+-dependent oxidation, and nicotinamide conversion is spectrophotometrically monitored by the change in UV absorbance at 340 nm
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-