5% loss of activity after 5 min in the presence of 0.2 mM pyridoxal 5'-phosphate and 2 mM 2-oxoglutarate, 60% loss of activity in the absence of cofactor and substrate after 5 min, more than 90% after 10 min
strain CB 60, complete inactivation after 8 min, 28% and 8% activity remain after 8 min in the presence of 2-oxoglutarate, strain CB 6, 68% loss of activity after 32 min, 35% and 22% loss of activity after 32 min in the presence of pyridoxal 5'-phosphate and 2-oxoglutarate respectively
holoenzyme, i.e. enzyme in its pyridoxal form, approx. 20% loss of phenylalanine transaminase activity after 6 h, apoenzyme, 70% loss of activity, complete protection of holoenzyme in the presence of pyridoxamine 5'-phosphate and 2-oxoglutarate
irreversible denaturation of the pure enzyme is not apparent after 6 h in the presence of pyridoxal 5'-phosphate and 2-ketoglutarate. The apoenzyme (lacking pyridoxal 5'-phosphate) loses 70% of its activity (measured after reconstitution) after 6 h