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2.4.1.B72: C-mannosyltransferase

This is an abbreviated version!
For detailed information about C-mannosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.B72

Reaction

transfers a D-mannosyl residue from dolichyl D-mannosyl phosphate to the tryptophan residue of a protein acceptor forming a C-C bond =

Synonyms

C-mannosyltransferase, DPY-19, Dpy-19-like C-mannosyltransferase, Dpy-19-like protein 3, dpy19, DPY19L1, DPY19L3, DPY19L4, dumpy-19, Dumpy-19 protein homologue, Pf DPY-19, PF3D7_0806200, protein dpy-19 homolog 3, protein dumpy-19

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.B72 C-mannosyltransferase

Cloned

Cloned on EC 2.4.1.B72 - C-mannosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene dpy-19, recombinant expression in Drosophila melanogaster S2 cells, coexpression with receptor proteins UNC-5 or MIG-21
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells
gene dpy19, recombinant enzyme expression in Drosophila melanogaster S2 cells
gene Dpy19l1, using CRISPR-Cas9 mutagenesis, Chinese hamster ovary (CHO) single knockout cells are generated as well as a triple knockout lacking DPY19L1, -L3, and -L4, and the respective mouse enzymes are reintroduced in the cells. Transient expression of C-terminal V5-tagged UNC5A in CHO cell single knockouts of DPY19L1, -L3, and -L4. Overexpression of DPY19L1 results in detection of TSR1 peptides with a maximum of two C-mannoses
gene Dpy19l3, using CRISPR-Cas9 mutagenesis, Chinese hamster ovary (CHO) single knockout cells are generated as well as a triple knockout lacking DPY19L1, -L3, and -L4, and the respective mouse enzymes are reintroduced in the cells. Transient expression of C-terminal V5-tagged UNC5A in CHO cell single knockouts of DPY19L1, -L3, and -L4. The ratio of this C-mannosylated peptide to the nonmannosylated peptide is higher in cells overexpressing DPY19L3 than in wild-type cells
gene Dpy19l4, using CRISPR-Cas9 mutagenesis, Chinese hamster ovary (CHO) single knockout cells are generated as well as a triple knockout lacking DPY19L1, -L3, and -L4, and the respective mouse enzymes are reintroduced in the cells. Transient expression of C-terminal V5-tagged UNC5A in CHO cell single knockouts of DPY19L1, -L3, and -L4
recombinant expression in S2 cells, coexpression with substrate Rspo1
transient recombinant expression of wild-type and mutant enzymes in Drosophila melanogaster S2 cells or HEK-293T cells fused to Gaussia luciferase or tagged with myc or myc-His6 tags, quantitative RT-PCR expression analysis