2.3.1.189: mycothiol synthase
This is an abbreviated version!
For detailed information about mycothiol synthase, go to the full flat file.
Word Map on EC 2.3.1.189
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2.3.1.189
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tuberculosis
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actinomycetes
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n-acetyltransferase
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smegmatis
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gnat
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amine
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gcn5-related
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thiol-disulfide
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accys-glcn-ins
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diagnostics
- 2.3.1.189
- tuberculosis
- actinomycetes
- n-acetyltransferase
- smegmatis
-
gnat
- amine
-
gcn5-related
-
thiol-disulfide
- accys-glcn-ins
- diagnostics
Reaction
Synonyms
MSH acetyltransferase, MSH synthase, MshD, nshD, Rv0819
ECTree
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Cloned
Cloned on EC 2.3.1.189 - mycothiol synthase
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cloned in pQE31 plasmid and transformed into Escherichia coli M15 (pREP4)
the Rv0819 gene encoding MshD (residues 1315) is amplified from Mycobacterium tuberculosis genomic DNA using PCR and cloned into the pET28a(+) plasmid. This vector construction adds six histidine residues and a thrombin cleavage sequence to the N-terminus to facilitate protein purification. Expression in Escherichia coli stain BL21(DE3)pLysS