Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.1.15: glycerol-3-phosphate 1-O-acyltransferase

This is an abbreviated version!
For detailed information about glycerol-3-phosphate 1-O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.15

Reaction

an acyl-CoA
+
sn-glycerol 3-phosphate
=
CoA
 +
a 1-acyl-sn-glycerol 3-phosphate

Synonyms

1-acyl-sn-glycerol-3-phosphate acyltransferase, 3-glycerophosphate acyltransferase, ACP:sn-glycerol-3-phosphate acyltransferase, acyl-CoA:glycerol-3-phosphate acyltransferase 1, acyl-CoA:sn-glycerol-3-phosphate O-acyltransferase, acyl-[acyl carrier protein]:sn-1-glycerol-3-phosphate acyltransferase, acyltransferase, glycerol phosphate, Ad-GPAT1, AGPAT, AGPAT10, AGPAT6, AGPAT8, AGPAT9, alpha-glycerophosphate acyltransferase, AT1G02390, AT1G06520, AT3G11430, AT4G00400, AT4G01950, AT5G06090, AT5G60620, AtGPAT1, AtGPAT2, AtGPAT3, AtGPAT5, AtGPAT7, AtGPAT8, AtGPAT9, cancer-testicular antigen, chloroplast glycerol-3-phosphate acyltransferase, CrGAPTcl, ER-associated GPAT, G3P acyltransferase, GAT, Gat1, GAT1p, GAT2p, glycerol 3-phosphate acyltransferase, glycerol phosphate acyltransferase, glycerol phosphate transacylase, glycerol-3-P acyltransferase 1, glycerol-3-phosphate acyltransferase, glycerol-3-phosphate acyltransferase 1, glycerol-3-phosphate acyltransferase 2, glycerol-3-phosphate acyltransferase 3, glycerol-3-phosphate acyltransferase 4, glycerol-3-phosphate acyltransferase 9, glycerol-3-phosphate acyltransferase-1, glycerol-3-phosphate acyltransferase-like protein 1, glycerol-3-phosphate O-acyltransferase, glycerol-3-phosphate-1 acyltransferase, glycerol-sn-3-phosphate acyltransferase 1, glycerophosphate acyltransferase, glycerophosphate transacylase, GPAM, GPAT, GPAT-1, GPAT-4, GPAT-9, GPAT1, GPAT2, GPAT3, GPAT3/AGPAT10, GPAT4, GPAT5, GPAT6, GPAT7, GPAT8, GPAT9, gpt1, Gpt2, LeGPAT, LiGPAT, LPAAT-theta, LPAAT-zeta, mGPAT, microsomal GPAT3, microsomal GPAT3/AGPAT10, microsomal GPAT4, mitochondrial glycerol-3-phosphate acyltransferase, mitochondrial GPAT1, mitochondrial GPAT2, More, MS33, mtGPAT, mtGPAT1, mtGPAT2, OsGPAT3, PHATRDRAFT_42446, PHYPADRAFT_137573, PLAT2, PlsB, PlsB1, Ppa GPAT9, PpGPAT9, PtGPAT, Rv1551, Rv1551 acyltransferase, SA1, Sn-glycerol 3-phosphate acyltransferase, sn-glycerol-3-phosphate acyltransferase, SsGPAT, TbGAT, water-soluble plastidial glycerol-3-phosphate acyltransferase, xGPAT1, xGPAT1-v1, xGPAT1-v2

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.15 glycerol-3-phosphate 1-O-acyltransferase

Cloned

Cloned on EC 2.3.1.15 - glycerol-3-phosphate 1-O-acyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a full length cDNA of the GPAT gene is subcloned into the expression vector pBI121, the gene is introduced into the tobacco, NC89, by Agrobacterium-mediated leaf disk transformation
chloroplast acyltransferase
cloning of cDNA
DNA and amino acid sequence determination and analysis, full functional complementation by expression of His6-tagged enzyme in an enzyme-deficient yeast mutant strain BYL118, the recombinant enzyme shows a slightly altered substrate specificity, overview
-
DNA and amino acid sequence determmination and analysis, phylogenetic analysis and tree, quantitative reverse-transcription real-time PCR expression analysis, functional complementation of the osgpat3 mutant, calluses induced from young panicles of the homozygous osgpat3 plants are used for transformation with Agrobacterium tumefaciens strain EHA105 carrying the pCAMBIA1301-OsGPAT3-GFP plasmid or the control plasmid pCAMBIA1301-GFP expressing GFP-tagged wild-type and mutant enzymes, map-based cloning and functional complementation of mutant osgpat3, localization of osgpat3 mutation on chromosome 11
elimination of isozyme mtGPAT1 in knockout mice
-
enzyme cloning and overexpression in Phaeodactylum tricornutum strain CCMP2561, PtGPAT cDNA is fused between the fucoxanthin chlorophyll a/c binding protein (fcp) fcpC promoter and fcpA terminator of Phaeodactylum tricornutum. Furthermore, an Omega leader sequence is added before the coding region of PtGPAT for enhancing its translation. A c-Myc tag is also fused to the C-terminal of PtGPAT, quantitative real-time RT-PCR expression analysis
expressed as a myc-epitope-tagged fusion protein in Tobacco suspension cultured cells
expressed in Escherichia coli XL1-Blue cells
expression in CHO-K1 cell
expression in Escherichia coli
expression in HEK-293 cell
expression of chimeric mature enzyme containing residues 128-187 from Spinacia oleracea and residues 1-127 from Cucurbita moscata, in Escherichia coli strain BL21(DE3)
-
expression of mitochondrial acyl transferase in Sf9 insect cells
-
expression of mitochondrial acyltransferase in Sf21 insect cells
expression of mitochondrial enzyme in CHO cells
-
expression of N-terminal truncated enzyme in Escherichia coli, overexpression of wild-type acyltransferase in tobacco
-
expression of native and selenomethionine-labeled wild-type and chimeric mature enzymes, the latter contains residues 128-187 from Spinacia oleracea and residues 1-127 from Cucurbita moscata, in Escherichia coli strain BL21(DE3) and a nonauxotrophic strain
-
expression of stromal acyltransferase in Escherichia coli
expression of wild-type and PCR-mutated cDNA in Escherichia coli
-
gene GPAT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa in Arabidopsis thaliana, quantitative real-time PCR expression analysis
-
gene GPAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expression analysis
gene GPAT2, sequence comparisons and phylogenetic tree, quantitative real-time PCR enzyme expression analysis
gene GPAT3, quantitative real-time PCR expression analysis
gene gpat3, real-time PCR enzyme expression analysis
gene GPAT4, quantitative real-time PCR expression analysis
gene GPAT9, recombinant expression in Saccharomyces cerevisiae strain gat1DELTA (Matalpha, his3C1, leu2C0, lys2C0, ura3C0, YKR067w::kanMX4), quantitative real-time RT-PCR analysis
gene LiGPAT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, detailed overview. Heterologous expression of wild-type mLiGPAT and mutant sdmLiGPAT R195H in the GPAT-deficient yeast mutant gat1DELTA. Recombinant expression of soluble His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS
gene MS33 or SA1, map-based cloning and gene structure of SA1/MS33, DNA and amino acid sequence determination and analysis, phylogenetic analysis of MS33, quantitative PCR enzyme expression analysis
gene plat2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of eYFP-tagged PpGPAT9 in Nicotiana benthamiana leaf epidermal cell endoplasmic reticulum using Agrobacterium tumefaciens transfection system. Recombinant ectopic expression of PpGPAT9 in Arabidopsis thaliana increasing the seed oil content by approximately 10%. The levels of polyunsaturated fatty acids (18:2, 18:3, and 20:2) and saturated fatty acids (16:0, 18:0, and 20:0) increase by 60% and 43%, respectively, in the storage oil of the transgenic seeds of Arabidopsis thaliana when compared with the wild-type, the transgenic embryos with increased oil content contain larger embryonic cells than the wild-type. Quantitative RT-PCR expression analysis
gene plat2, DNA and amino acid sequence determination and analysis, the sequence of plat2 from Aurantiochytrium limacinum strain ATCC MYA-1381 is used for screening, sequence comparisons and phylogenetic tree, recombinant overexpression in Saccharoymces cerevisiae strain INVSc1, functional recombinant overexpression of FLAG-tagged enzyme in Aurantiochytrium limacinum strain F26-b, subcloning in Escherichia coli strain JM109
gene plsB1 or Rv1551, DNA and amino acid sequence determination and analysis, sequence comparisons, subcloning in Escherichia coli strain TOP10, functional recombinant expression in Escherichia coli strain BL21 Star (DE3) leading to significantly (2-4fold) higher viable cell counts during the exponential and stationary phases. Growth of Escherichia coli in medium containing long-chain fatty acids is enhanced by mGPAT. The enzyme displays function as a GPAT by enhancing the synthesis of phospholipids from exogenously provided fatty acids in Escherichia coli cell lysates
gene Tb10.70.4400, sequence comaprisons, TbGAT restores glycerol-3-phosphate acyltransferase activity when expressed in a Leishmania major deletion strain lacking this activity and exhibits preference for medium length, unsaturated fatty acyl-CoAs
GPAT3 is localized to the endoplasmic reticulum when overexpressed in COS-7 cells
GPAT3 overexpression in COS-7 cells
hepatic overexpression of the enzyme with an adenoviral construct
-
into the mammalian expression vector p3xFLAG-CMV-14 for transfection of HEK-293 cells
-
into the pBI121 vector for introducing the construct into Agrobacterium tumefaciens for subsequent transformation of tomato plants
into the pGEM12zf+ vector
-
into the pMD-18T vector for sequencing and subcloned into the pBI121 vector for introducing the construct into Agrobacterium tumefaciens for subsequent transformation of tomato plants
into the pMD-18T vector for sequencing, into the p35S-GFP vector for intracellular targeting, into the pET30a+ vector for expression in Escherichia coli BL21 cells, into the pBI121 vector for Agrobacterium-mediated transformation of tomato plants
mice are transduced with adenoviruses expressing either murine mtGPAT or a catalytically inactive variant of the enzyme. Overexpressing mtGPAT results in massive 12- and 7-fold accumulation of liver triacylglycerol and diacylglycerol
mutant enzymes expressed in CHO cells
-
overexpression in tobacco
-
overexpression of FLAG-tagged mitochondrial isozymes in HEK293 cells and in primary hepatocytes from fasted rats via adenovirus transfection system, leading to increased enzyme activity and incorporation of added oleate into triacylglycerol, the triacylgycerol is secreted from the recombinant hepatocytes if higher concentrated, phospholipid composition in hepatocytes, overview
-
partial-length cDNA of CrGPATcl with the omitted coding sequence for the predicted chloroplast transit peptide (i.e. residues 1-20) is amplified from a cDNA library of Chlamydomonas reinhardtii, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain M15
PCR amplified mouse testis cDNA is cloned into the TOPO vector and subsequently subcloned into pcDNA3.1 for transient transfection of COS-7 and HEK293 cells
-
plsB structural gene encoding glycerol 3-phosphate acyltransferase
-
recombinant GPAT1-FLAG adenovirus and Ad-EGFP are constructed
-
the PCR product is subcloned with the TOPO TA Cloning Kit and sequenced, the vectors pCR2.1-TOPO and pcDNA3.1/Myc-His+ are used, the xGPAT-myc construct is used for transfection of CHO-K1 cells
the promotor regions, distal and proximal, are cloned into the promotorless mammalian expression vector pGL3-basic that contains firefly luciferase as the reporter gene
transient expression of epitope-tagged acyltransferase in CHO K1 cells
-