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2.1.1.257: tRNA (pseudouridine54-N1)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (pseudouridine54-N1)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.257

Reaction

S-adenosyl-L-methionine
+
pseudouridine54 in tRNA
=
S-adenosyl-L-homocysteine
 +
N1-methylpseudouridine54 in tRNA

Synonyms

COG 1901, COG1901, DUF358, DUF358/Mj1640, Emg1, Hvo_1989, m1-pseudouridine methyltransferase, m1G, m1PSI methyltransferase, M3U, Mja_1640, MjNep1, Nep1, Pfam DUF358, pseudouridine N1-specific methyltransferase, pseudouridine-N1-specific methyltransferase, ribosome assembly factor Nep1, SAM-dependent N1-pseudouridine methyltransferase, TrmY, TrmY protein

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.257 tRNA (pseudouridine54-N1)-methyltransferase

Cloned

Cloned on EC 2.1.1.257 - tRNA (pseudouridine54-N1)-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Mj1640, expression of wild-type and selenmethionine-labeled enzyme as N-terminally His-tagged protein in Escherichia coli strain Bl21(DE3)
gene trmY or HVO_1989
gene VNG1980C, expression of the gene encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain