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88 - 90
2-deoxy-D-glucose
2 - 46
3-O-methyl-D-glucose
additional information
additional information
-
under conditions that avoid its masking by sugar-substrate inhibition as much as possible, negative kinetic cooperativity with respect to sugar substrate oxidation is observed. The cooperativity effect dramatically changes the performance of soluble GDH, as reflected by the V2 and K2 values for glucose in phosphate buffer being about 10-fold and 100-fold higher than the V1 and K1 values, respectively. Substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose
-
88
2-deoxy-D-glucose
-
mutant E277K, pH 7.0
90
2-deoxy-D-glucose
-
wild-type, pH 7.0
2 - 3
3-O-methyl-D-glucose
-
recombinant wild-type with R4-tail, pH 7.0
22
3-O-methyl-D-glucose
-
heterodimer wild-type/mutant H168Q, pH 7.0
27
3-O-methyl-D-glucose
-
mutant E277K, pH 7.0
27.6
3-O-methyl-D-glucose
-
mutant N452T, pH 7.0
28.7
3-O-methyl-D-glucose
-
wild-type, pH 7.0
28.8
3-O-methyl-D-glucose
-
mutant N462H, pH 7.0
46
3-O-methyl-D-glucose
-
wild-type, pH 7.0
21
D-allose
-
mutant E277K, pH 7.0
29
D-allose
-
wild-type, pH 7.0
32.5
D-allose
-
mutant N462H, pH 7.0
34
D-allose
-
recombinant wild-type with R4-tail, pH 7.0
35.5
D-allose
-
wild-type, pH 7.0
36
D-allose
-
heterodimer wild-type/mutant H168Q, pH 7.0
38.7
D-allose
-
mutant N452T, pH 7.0
2.7
D-galactose
-
mutant N462H, pH 7.0
3.7
D-galactose
-
mutant N452T, pH 7.0
5.3
D-galactose
-
wild-type, pH 7.0
6.8
D-galactose
-
wild-type, pH 7.0
6.8
D-galactose
-
mutant E277K, pH 7.0
8
D-galactose
-
recombinant wild-type with R4-tail, pH 7.0
9
D-galactose
-
heterodimer wild-type/mutant H168Q, pH 7.0
0.74
D-glucose
pH and temperature not specified in the publication
2 - 3
D-glucose
-
recombinant wild-type with R4-tail, pH 7.0
4.91
D-glucose
-
at pH 7.0 and 25°C
8.8
D-glucose
-
mutant E277K, pH 7.0
12.3
D-glucose
-
mutant N462H, pH 7.0
12.5
D-glucose
-
mutant N452T, pH 7.0
16
D-glucose
-
mutant T416V/T417V, pH 7.0, 25°C
17.5
D-glucose
-
recombinant enzyme, expression in Pichia pastoris
20
D-glucose
-
wild-type, pH 7.0, 25°C
20
D-glucose
-
mutant D340F/Y418F, pH 7.0, 25°C
20
D-glucose
-
mutant D340F/Y418I, pH 7.0, 25°C
22
D-glucose
-
heterodimer wild-type/mutant H168Q, pH 7.0
25
D-glucose
-
wild-type, pH 7.0
25
D-glucose
-
recombinant enzyme, expression in Escherichia coli
26.8
D-glucose
-
wild-type, pH 7.0
27
D-glucose
-
mutant S231K, pH 7.0
43
D-glucose
wild type enzyme, at pH 7.0 and 37°C
64
D-glucose
mutant enzyme N428C, at pH 7.0 and 37°C
14.3
D-lactose
-
wild-type, pH 7.0
18
D-lactose
-
mutant N462H, pH 7.0
18.9
D-lactose
-
wild-type, pH 7.0
33.6
D-lactose
-
mutant N452T, pH 7.0
34
D-lactose
-
mutant E277K, pH 7.0
14.3
D-maltose
-
mutant E277K, pH 7.0
16
D-maltose
-
mutant N462H, pH 7.0
26
D-maltose
-
wild-type, pH 7.0
30.9
D-maltose
-
wild-type, pH 7.0
46.5
D-maltose
-
mutant N452T, pH 7.0
22
D-mannose
-
wild-type, pH 7.0
22
D-mannose
-
mutant E277K, pH 7.0
7.5
D-xylose
-
mutant E277K, pH 7.0
14.3
D-xylose
-
wild-type, pH 7.0
0.0014
lactose
electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 8.0, temperature not specified in the publication
0.0018
lactose
electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 7.0, temperature not specified in the publication
0.0019
lactose
electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 8.0, temperature not specified in the publication
0.0023
lactose
electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 7.0, temperature not specified in the publication
17.5
lactose
-
recombinant enzyme, expression in Pichia pastoris
18.9
lactose
-
recombinant enzyme, expression in Escherichia coli
20
lactose
-
heterodimer wild-type/mutant H168Q, pH 7.0
20
lactose
-
recombinant wild-type with R4-tail, pH 7.0
13
maltose
-
heterodimer wild-type/mutant H168Q, pH 7.0
14
maltose
-
recombinant wild-type with R4-tail, pH 7.0