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2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
cellobiose + N-methylphenazonium methylsulfate
cellobiono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
cellobiose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-allose + N-methylphenazonium methylsulfate
D-allono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-fucose + oxidized 2,6-dichlorophenolindophenol
D-fucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-galactose + N-methylphenazonium methylsulfate
D-galactono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
30% activity compared to D-glucose
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-galactose + oxidized N-methylphenazonium methyl sulfate
D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
D-glucose + dimethyl((6-methyl-7-((4-nitrosophenyl)amino)-1H-imidazo[1,2-b]pyrazol-1-yl)methyl)phosphine oxide
D-glucono-1,5-lactone + reduced dimethyl((6-methyl-7-((4-nitrosophenyl)amino)-1H-imidazo[1,2-b]pyrazol-1-yl)methyl)phosphine oxide
-
-
-
?
D-glucose + N-methylphenazonium methylsulfate
D-glucono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
100% activity
-
-
?
D-glucose + osmium polymer
D-glucono-1,5-lactone + reduced osmium polymer
-
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenol-indophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-glucose + oxidized N-methylphenazonium methyl sulfate
D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + reduced phenazine methosulfate
D-lyxose + N-methylphenazonium methylsulfate
D-lyxono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
D-lyxose + oxidized 2,6-dichlorophenolindophenol
D-lyxono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-mannose + N-methylphenazonium methylsulfate
D-mannono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-ribose + N-methylphenazonium methylsulfate
D-ribono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
5% activity compared to D-glucose
-
-
?
D-ribose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-xylose + N-methylphenazonium methylsulfate
D-xylono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
14% activity compared to D-glucose
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-xylose + oxidized N-methylphenazonium methyl sulfate
D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
-
?
L-arabinose + N-methylphenazonium methylsulfate
L-arabinono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
L-arabinose + oxidized 2,6-dichlorophenolindophenol
L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
lactose + 1-(N,N-dimethylamine)-4-(4-morpholine)benzene
lactono-1,5-lactone + reduced 1-(N,N-dimethylamine)-4-(4-morpholine)benzene
-
-
-
?
lactose + N,N'-dimethyl-4,4'-azopyridinium methyl sulfate
lactono-1,5-lactone + reduced N,N'-dimethyl-4,4'-azopyridinium methyl sulfate
-
-
-
?
lactose + N-methylphenazonium methylsulfate
lactono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
4% activity compared to D-glucose
-
-
?
lactose + oxidized 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
lactose + oxidized 2,6-dichlorophenolindophenol
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
lactose + oxidized N-methylphenazonium methyl sulfate
4-O-beta-D-galactopyranosyl-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
-
?
maltose + acceptor
maltono-1,5-lactone + reduced acceptor
-
-
-
?
maltose + N-methylphenazonium methylsulfate
maltono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
12% activity compared to D-glucose
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
maltose + oxidized 2,6-dichlorophenolindophenol
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
melibiose + N-methylphenazonium methylsulfate
melibiono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
5% activity compared to D-glucose
-
-
?
melibiose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
additional information
?
-
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
4% of the activity with D-glucose
-
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
81% of the activity with D-glucose
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
47% of the activity with D-glucose
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
11% of the activity with D-glucose
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 13% activity compared to D-glucose
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 13% activity compared to D-glucose
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
100% activity
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
100% activity
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + reduced phenazine methosulfate
-
-
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + reduced phenazine methosulfate
-
-
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + reduced phenazine methosulfate
-
-
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + reduced phenazine methosulfate
-
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
13% of the activity with D-glucose
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 11% activity compared to D-glucose
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
7% of the activity with D-glucose
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 9% activity compared to D-glucose
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
61% of the activity with D-glucose
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
61% of the activity with D-glucose
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 50% activity compared to D-glucose
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
61% of the activity with D-glucose
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
61% of the activity with D-glucose
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 70% activity compared to D-glucose
-
-
?
additional information
?
-
-
kinetic mechanism of sGDH consists of (a) step(s) in which a fluorescing intermediate is formed, and a subsequent, irreversible step, determining the overall rate of the reductive half-reaction. The pH optimum determined for glucose oxidation appears to be 7.0, implying that reoxidation of sGDHred is rate-limiting with those electron acceptors displaying a different value under steady-state conditions.
-
-
?
additional information
?
-
-
mechanism comprises general base-catalyzed hydride transfer
-
-
?
additional information
?
-
-
the sequential steps in the mechanism of sGDH must be reversible substrate binding, direct transfer of a hydride ion (reversible or irreversible) from the C1 position of the beta-anomer of glucose to the C5 of PQQ, irreversible, rate-determining tautomerization of the fluorescing, C5-reduced PQQ to PQQH2 and release (or earlier) of the product, D-glucono-delta-lactone, and oxidation of PQQH2 by an electron acceptor. The PQQ-activating Ca2+ greatly facilitates the reactions occurring in the second step. His144 may also play a role in this by acting as a general base catalyst, initiating hydride transfer by abstracting a proton from the anomeric OH group of glucose
-
-
?
additional information
?
-
-
almost no activity with D-fructose
-
-
?
additional information
?
-
-
no activity with D-glucosamine, L-altrose, L-lyxose, L-talose, L-mannose, D-altrose, D-arabinose, D-talose, L-xylose, L-glucose, L-galactose, L-allose, sucrose, trehalose, and raffinose
-
-
?
additional information
?
-
-
almost no activity with D-fructose
-
-
?
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D275E
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 11%, D-allose 44%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 7%, D-lactose 52%, D-maltose 44%, respectively
D276E
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 13%, D-mannose 19%, D-allose 54%, 3-O-methyl-D-glucose 57%, D-galactose 26%, D-xylose 20%, D-lactose 75%, D-maltose 66%, respectively
D340F/Y418F
-
increase in temperature stability
D340F/Y418I
-
increase in temperature stability
D448N
-
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 72%, D-allose 39%, D-galactose 14%, D-maltose 36%, D-lactose 48%, respectively
D456N
-
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 78%, D-allose 43%, D-galactose 16%, D-maltose 41%, D-lactose 59%, respectively
DD457N
-
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 80%, D-allose 46%, D-galactose 11%, D-maltose 32%, D-lactose 43%, respectively
E277A
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 11%, D-mannose 33%, D-allose 142%, 3-O-methyl-D-glucose 91%, D-galactose 46%, D-xylose 23%, D-lactose 83%, D-maltose 39%, respectively
E277D
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 9%, D-mannose 19%, D-allose 68%, 3-O-methyl-D-glucose 58%, D-galactose 21%, D-xylose 15%, D-lactose 87%, D-maltose 73%, respectively
E277G
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 15%, D-mannose 38%, D-allose 189%, 3-O-methyl-D-glucose 113%, D-galactose 59%, D-xylose 28%, D-lactose 100%, D-maltose 52%, respectively
E277H
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 11%, D-allose 67%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 9%, D-lactose 57%, D-maltose 39%, respectively
E277K
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 7%, D-mannose 18%, D-allose 116%, 3-O-methyl-D-glucose 84%, D-galactose 28%, D-xylose 18%, D-lactose 79%, D-maltose 74%, respectively
E277N
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 148%, 3-O-methyl-D-glucose 91%, D-galactose 43%, D-xylose 18%, D-lactose 86%, D-maltose 59%, respectively
E277Q
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 10%, D-mannose 31%, D-allose 134%, 3-O-methyl-D-glucose 80%, D-galactose 35%, D-xylose 25%, D-lactose 68%, D-maltose 54%, respectively
E277V
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 150%, 3-O-methyl-D-glucose 101%, D-galactose 25%, D-xylose 19%, D-lactose 114%, D-maltose 65%, respectively
I278F
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 4%, D-mannose 14%, D-allose 54%, 3-O-methyl-D-glucose 47%, D-galactose 15%, D-xylose 12%, D-lactose 64%, D-maltose 49%, respectively
N279H
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 6%, D-allose 49%, 3-O-methyl-D-glucose 50%, D-galactose 13%, D-xylose 8%, D-lactose 64%, D-maltose 61%, respectively
N452T
-
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 59%, D-allose 33%, D-galactose 5%, D-maltose 30%, D-lactose 31%, respectively
N462H
-
substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 53%, D-allose 32%, D-galactose 2%, D-maltose 25%, D-lactose 31%, respectively
S231C
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 8%, D-allose 46%, 3-O-methyl-D-glucose 76%, D-galactose 14%, D-xylose 8%, D-lactose 69%, D-maltose 69%, respectively
S231D
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 9%, D-allose 38%, 3-O-methyl-D-glucose 71%, D-galactose 12%, D-xylose 8%, D-lactose 54%, D-maltose 38%, respectively
S231H
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 12%, D-allose 57%, 3-O-methyl-D-glucose 86%, D-galactose 17%, D-xylose 7%, D-lactose 56%, D-maltose 38%, respectively
S231K
-
increase in thermal stability. Substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 10%, D-allose 43%, 3-O-methyl-D-glucose 82%, D-galactose 15%, D-xylose 5%, D-lactose 59%, D-maltose 70%, respectively
S231L
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 13%, D-allose 62%, 3-O-methyl-D-glucose 105%, D-galactose 20%, D-xylose 12%, D-lactose 73%, D-maltose 76%, respectively
S231M
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 9%, D-allose 43%, 3-O-methyl-D-glucose 80%, D-galactose 10%, D-xylose 8%, D-lactose 56%, D-maltose 41%, respectively
S231N
-
substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 12%, D-allose 61%, 3-O-methyl-D-glucose 109%, D-galactose 18%, D-xylose 15%, D-lactose 66%, D-maltose 51%, respectively
T416V/T417V
-
increase in temperature stability
N428C
the catalytic efficiency is increased by 47% compared to the wild type enzyme, the mutant shows increased affinity for pyrroloquinoline quinone and is twice more active toward D-glucose and more selective toward maltose than the wild type
N428C
the mutant shows almost twice the maximum catalytic activity compared to the wild type
additional information
-
constructed of heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits. The heterodimeric wild-type/H168Q shows slightly decreased GDH activity and almost identical substrate specificity profile to the wild-type enzyme. The Hill coefficient of the heterodimer is calculated as 1.13, indicating positive cooperativity
additional information
-
expression in Pichia pastoris using the Saccharomyces cerevisiae alpha-factor signal sequence for secretion. The productivity of secreted PQQGDH-B achieves 218 kU/liter, i.e. 43 mg/liter. The secreted PQQGDH-B in Pichia pastoris is glycosylated but shows similar enzymatic properties as compared with those of recombinant PQQGDH-B produced in Escherichia coli
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Oubrie, A.; Rozeboom, H.J.; Kalk, K.H.; Duine, J.A.; Dijkstra, B.W.
The 1.7 A crystal structure of the apo form of the soluble quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus reveals a novel internal conserved sequence repeat
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Acinetobacter calcoaceticus
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Igarashi, S.; Ohtera, T.; Yoshida, H.; Witarto, A.B.; Sode, K.
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Acinetobacter calcoaceticus
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Acinetobacter calcoaceticus
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Acinetobacter calcoaceticus
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Acinetobacter calcoaceticus
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Oubrie, A.
Structure and mechanism of soluble glucose dehydrogenase and other PQQ-dependent enzymes
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Construction and characterization of heterodimeric soluble quinoprotein glucose dehydrogenase
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Acinetobacter calcoaceticus
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Ca2+-assisted, direct hydride transfer, and rate-determining tautomerization of C5-reduced PQQ to PQQH2, in the oxidation of beta -D-glucose by soluble, quinoprotein glucose dehydrogenase
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Acinetobacter calcoaceticus
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Matsushita, K.; Toyama, H.; Ameyama, M.; Adachi, O.; Dewanti, A.; Duine, J.A.
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Kojima, K.; Witarto, A.B.; Sode, K.
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Acinetobacter calcoaceticus
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Olsthoorn, A.J.J.; Otsuki, T.; Duine, J.A.
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Efficient direct electron transfer of PQQ-glucose dehydrogenase on carbon cryogel electrodes at neutral pH
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Acinetobacter calcoaceticus (P13650), Acinetobacter calcoaceticus
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Durand, F.; Stines-Chaumeil, C.; Flexer, V.; Andre, I.; Mano, N.
Designing a highly active soluble PQQ-glucose dehydrogenase for efficient glucose biosensors and biofuel cells
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Acinetobacter calcoaceticus (P13650)
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Schubart, I.; Gbel, G.; Lisdat, F.
A pyrroloquinolinequinone-dependent glucose dehydrogenase (PQQ-GDH)-electrode with direct electron transfer based on polyaniline modified carbon nanotubes for biofuel cell application
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Yu, Y.; Wei, P.; Zhu, X.; Huang, L.; Cai, J.; Xu, Z.
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Wired pyrroloquinoline quinone soluble glucose dehydrogenase enzyme electrodes operating at unprecedented low redox potential
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Acinetobacter calcoaceticus
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Coexpression of the pyrroloquinoline quinone and glucose dehydrogenase genes from Serratia marcescens CTM 50650 conferred high mineral phosphate-solubilizing ability to Escherichia coli
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Serratia marcescens, Serratia marcescens CTM 50650
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Stredansky, M.; Monosik, R.; Mastihuba, V.; Sturdik, E.
Monitoring of PQQ-dependent glucose dehydrogenase substrate specificity for its potential use in biocatalysis and bioanalysis
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Acinetobacter calcoaceticus
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An, R.; Moe, L.A.
Regulation of pyrroloquinoline quinone-dependent glucose dehydrogenase activity in the model rhizosphere-dwelling bacterium Pseudomonas putida KT2440
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Pseudomonas putida, Pseudomonas putida KT 2240
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Bharwad, K.; Rajkumar, S.
Modulation of PQQ-dependent glucose dehydrogenase (mGDH and sGDH) activity by succinate in phosphate solubilizing plant growth promoting Acinetobacter sp. SK2
3 Biotech
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5
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Acinetobacter sp.
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Stines-Chaumeil, C.; Mavre, F.; Kauffmann, B.; Mano, N.; Limoges, B.
Mechanism of reconstitution/activation of the soluble PQQ-dependent glucose dehydrogenase from Acinetobacter calcoaceticus a comprehensive study
ACS Omega
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2015-2026
2020
Acinetobacter calcoaceticus (P05465)
brenda
Lisdat, F.
PQQ-GDH - Structure, function and application in bioelectrochemistry
Bioelectrochemistry
134
107496
2020
Acinetobacter calcoaceticus (P05465)
brenda
Fusco, G.; Goebel, G.; Zanoni, R.; Bracciale, M.P.; Favero, G.; Mazzei, F.; Lisdat, F.
Aqueous polythiophene electrosynthesis A new route to an efficient electrode coupling of PQQ-dependent glucose dehydrogenase for sensing and bioenergetic applications
Biosens. Bioelectron.
112
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Acinetobacter calcoaceticus (P05465)
brenda
Vaitkute, G.; Bratkovskaja, I.; Casaite, V.; Stankeviciute, J.; Meskys, R.; Tetianec, L.
Electron transfer mediators for PQQ dependent soluble glucose dehydrogenase catalyzed lactose oxidation reaction
Chemija
30
194-200
2019
Acinetobacter calcoaceticus (P05465)
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brenda