EC Number   |
Protein Variants |
Reference |
|---|
   2.3.1.7 | D356A/M564G |
shows 6fold higher activity toward palmitoyl-CoA than that of the single mutant M564G and a new activity toward stearoyl-CoA. Mutations convert the enzyme into a pseudo carnitine palmitoyltransferase in terms of substrate specificity |
672172 |
| 2.3.1.7 | E347A |
complete loss of activity |
659372 |
| 2.3.1.7 | F545A |
increases Km for both substrates |
659815 |
| 2.3.1.7 | F545Y |
minor effects on Km for both substrates |
659815 |
| 2.3.1.7 | F565A |
used for crystallization, only minor effect on protein structure, increased activity with acetyl-CoA |
659365 |
| 2.3.1.7 | H343A |
complete loss of activity |
659372 |
| 2.3.1.7 | H343A |
mutant enzymje shows no activity with acetyl-CoA and hexanoyl-CoA |
674651 |
| 2.3.1.7 | H343E |
kcat/KM for acetyl-CoA is 9.8fold lower than wild-type value, mutant enzyme shows mo activity with hexanoyl-CoA as substrate |
674651 |
| 2.3.1.7 | M564A |
lowered activity with acetyl-CoA, increased activity with longer chain acyl-CoAs |
659372 |
| 2.3.1.7 | M564A |
used for crystallization, decreased activity with acetyl-CoA and butyryl-CoA |
659365 |
