EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.145 | C111A |
contrary to wild-type, presence of 2-mercaptoethanol does not decrease Km value for substrate and NAD+ |
688685 |
| 1.1.1.145 | C111S |
contrary to wild-type, presence of 2-mercaptoethanol does not decrease Km value for substrate and NAD+ |
688685 |
| 1.1.1.145 | C72F |
contrary to wild-type, presence of 2-mercaptoethanol does not decrease Km value for substrate and NAD+ |
688685 |
| 1.1.1.145 | C72S |
contrary to wild-type, presence of 2-mercaptoethanol does not decrease Km value for substrate and NAD+ |
688685 |
| 1.1.1.145 | C83A |
2-5fold increase in Km values |
688685 |
| 1.1.1.145 | C83A |
mutation produces fully active enzyme with elevated Km values for ligands |
763756 |
| 1.1.1.145 | C83S |
mutation in the active site abolishes the dehydrogenase and isomerase activities of the enzyme |
763756 |
| 1.1.1.145 | C83S |
no catalytic acitivity |
688685 |
| 1.1.1.145 | C83S |
similar to wild-type, presence of 2-mercaptoethanol results in 2.5fold decrease of Km values |
688685 |
| 1.1.1.145 | D241N |
39% of wild-type activity |
656788 |
