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Literature summary for 1.1.1.145 extracted from

  • Thomas, J.L.; Duax, W.L.; Addlagatta, A.; Kacsoh, B.; Brandt, S.E.; Norris, W.B.
    Structure/function aspects of human 3beta-hydroxysteroid dehydrogenase (2004), Mol. Cell. Endocrinol., 215, 73-82.
    View publication on PubMed
Search for more literature for 1.1.1.145

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cells Homo sapiens
expression in Sf9 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D241N 39% of wild-type activity Homo sapiens
D257L trace activity Homo sapiens
D258L trace activity Homo sapiens
D265N 12% of wild-type activity Homo sapiens
D36A/K37R uses NADP+ as cofactor, no activity with NAD+ Homo sapiens
H156Y 195% of wild-type activity Homo sapiens
K158Q no activity Homo sapiens
K273Q no activity Homo sapiens
Y154F no activity Homo sapiens
Y269S no activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
epostane
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 dehydroepiandrosterone 27°C, pH 7.4 Homo sapiens
0.025
-
 NAD+ 27°C, pH 7.4, H156Y mutant Homo sapiens
0.034
-
 NAD+ 27°C, pH 7.4 Homo sapiens
0.042
-
 dehydroepiandrosterone 27°C, pH 7.4, H156Y mutant Homo sapiens
0.047
-
 dehydroepiandrosterone 27°C, pH 7.4 Homo sapiens
0.086
-
 NAD+ 27°C, pH 7.4 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE, recombinant 3beta-HSD Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dehydroepiandrosterone + NAD+ Homo sapiens
-
 androst-5-en-3,17-dione + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant 3beta-HSD Homo sapiens
recombinant 3beta-HSD type 2 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
 Homo sapiens
-
gonad
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroepiandrosterone + NAD+
-
 Homo sapiens androst-5-en-3,17-dione + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE, recombinant 3beta-HSD Homo sapiens

Synonyms

Synonyms Comment Organism
3beta-hydroxysteroid dehydrogenase type 1
-
 Homo sapiens
3beta-hydroxysteroid dehydrogenase type 2
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00007
-
 epostane 27°C, pH 7.4 Homo sapiens
0.00098
-
 epostane 27°C, pH 7.4 Homo sapiens