EC Number |
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1.1.1.1 | - |
1.1.1.1 | 1 A resolution crystal structures of liver alcohol dehydrogenase in complex with NADH and two inhibitors: dimethyl sulfoxide and isobutyramide |
1.1.1.1 | 10 mg/ml purified double mutant H51Q/K228R in complex with NAD+ and 2,3- or 2,4-difluorobenzyl alcohol, in 50 mM ammonium N-[tris(hydroxymethyl)-methyl]-2-aminoethanesulfonate, pH 7.0, 5°C, 1 mM NAD+, 10 mM 2,3-difluorobenzyl alcohol or 2,4-difluorobenzyl alcohol, equilibrated against increasing concentrations of 2-methyl-2,4-pentanediole, crystal formation at 12% 2-methyl-2,4-pentanediole, X-ray diffraction structure determination and analysis |
1.1.1.1 | apoenzyme and ternary complex of enzyme with NADH and 2-ethoxyethanol bound to each subunit, X-ray diffraction structure determination and analysis at 2.3 A resolution |
1.1.1.1 | crystals are grown in the Advanced Protein Crystallization Facility during the Life and Microgravity Sciences Spacelab mission on the US Space Shuttle. Large diffracting crystals are obtained by dialysis, whereas only poor-quality crystals are obtained by vapour diffusion. The quality of both the microgravity and ground-based crystals is analysed by X-ray diffraction. There is some improvement in terms of size and diffraction resolution limit for the microgravity crystals. The twinning observed in the Earthgrown crystals is also present for those grown in microgravity |
1.1.1.1 | enzyme in complex with substrates NADH or NAD+, and 4-methylbenzyl alcohol, or with NAD+ and 4-bromobenzyl alcohol or 1H,1H-heptafluorobutanol, X-ray diffraction structure determination and analysis |
1.1.1.1 | enzyme in complex with trifluoroethanol and without NAD+, X-ray diffraction structure determination and analysis at 2.35 A resolution |
1.1.1.1 | enzyme-NADP+-cofactor complex, X-ray diffraction strcuture determination and analysis, computational structure modeling |
1.1.1.1 | holo-enzyme form and apo-enzyme form |
1.1.1.1 | isozyme alphaalpha in complex with inhibitor N-cyclopentyl-N-cyclobutylformamide, isozyme beta(1)beta(1) in complex with inhibitors N-benzylformamide and N-heptylformamide, and isozyme gamma(2)gamma(2) in complex with inhibitor N-1-methylheptylformamide, X-ray diffraction structure determination and analysis at 1.45-2.5 A resolution, structure modeling |