EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.1.1.9 | -999 |
- |
wild-type: half denaturation temperature T1/2 (°C): 35.2, thermal transition temperature Tcd (°C): 43.0 |
687872 |
1.1.1.9 | 0 |
40 |
10 min, 2.5 mM NAD+, stable |
686433 |
1.1.1.9 | 20 |
- |
at room temperature, the enzyme is inactivated within 2 days |
686433 |
1.1.1.9 | 25 |
- |
and below stable |
246421 |
1.1.1.9 | 30 |
- |
1 h, completely stable |
655092 |
1.1.1.9 | 30 |
45 |
the enzyme exhibits activities of approximately 81% and 51% after being incubated at 30°C and 35°C, respectively, for 30 min. The enzyme completely loses its activity at temperatures greater than 45°C |
738728 |
1.1.1.9 | 40 |
- |
half-life 120 min |
710975 |
1.1.1.9 | 45 |
50 |
approximately 92% and 70% activities remain following incubations at 45 and 50°C, respectively. The enzyme completely loses its activity above 60°C |
737866 |
1.1.1.9 | 45 |
60 |
the enzyme partially loses its activity at 45-55°C and completely loses activity at temperatures greater than 60°C. McXDH exhibits activities of approximately 81% and 51% at 30°C and 35°C after 30 min incubation |
761551 |
1.1.1.9 | 50 |
- |
1 h, no residual activity |
655092 |