Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.9 extracted from

  • Annaluru, N.; Watanabe, S.; Pack, S.P.; Saleh, A.A.; Kodaki, T.; Makino, K.
    Thermostabilization of Pichia stipitis xylitol dehydrogenase by mutation of structural zinc-binding loop (2007), J. Biotechnol., 129, 717-722.
    View publication on PubMed

Application

Application Comment Organism
molecular biology a high thermostability of PsXDH is obtained by subsequent site-directed mutagenesis of the structural zinc-binding loop. The best mutant in this study (C4/F98R/E101F) shows a 10.8 °C higher thermal transition temperature and 20.8 °C higher half denaturation temperature (T1/2) compared with wild-type Scheffersomyces stipitis

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Scheffersomyces stipitis

Protein Variants

Protein Variants Comment Organism
S96C/S99C/Y102C specific activity (U/min): 1440, half denaturation temperature T1/2 (°C): 46.1, thermal transition temperature Tcd (°C): 47.5 Scheffersomyces stipitis
S96C/S99C/Y102C/E101F specific activity (U/min): 1550, half denaturation temperature T1/2 (°C): 50.9, thermal transition temperature Tcd (°C): 50.5 Scheffersomyces stipitis
S96C/S99C/Y102C/F98R specific activity (U/min): 1510, half denaturation temperature T1/2 (°C): 53.1, thermal transition temperature Tcd (°C): 51.7 Scheffersomyces stipitis
S96C/S99C/Y102C/F98R/E101F specific activity (U/min): 1620, half denaturation temperature T1/2 (°C): 56.0, thermal transition temperature Tcd (°C): 53.8 Scheffersomyces stipitis
S96C/S99C/Y102C/H112D specific activity (U/min): 1360, half denaturation temperature T1/2 (°C): 44.0, thermal transition temperature Tcd (°C): 47.0 Scheffersomyces stipitis
S96C/S99C/Y102C/P95S specific activity (U/min): 1220, half denaturation temperature T1/2 (°C): 37.4, thermal transition temperature Tcd (°C): 43.5 Scheffersomyces stipitis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Scheffersomyces stipitis

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity (U/min): 1110 (wild-type), 1440 (mutant S96C/S99C/Y102C), 1220 (mutant S96C/S99C/Y102C/P95S), 1510 (mutant S96C/S99C/Y102C/F98R), 1550 (mutant S96C/S99C/Y102C/E101F), 1360 (mutant S96C/S99C/Y102C/H112D), 1620 (mutant S96C/S99C/Y102C/F98R/E101F) Scheffersomyces stipitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
Scheffersomyces stipitis D-xylulose + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
PsXDH
-
Scheffersomyces stipitis
xylitol dehydrogenase
-
Scheffersomyces stipitis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Scheffersomyces stipitis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
wild-type: half denaturation temperature T1/2 (°C): 35.2, thermal transition temperature Tcd (°C): 43.0 Scheffersomyces stipitis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Scheffersomyces stipitis