EC Number   |
Application |
Reference |
|---|
   2.7.1.78 | analysis |
a method for real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by use of a singly fluorophore-labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage is described |
695561 |
| 2.7.1.78 | analysis |
assay for the detection of T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy. The method is highly sensitive, the T4 PNKP detection limit is 0.01 U/ml |
749573 |
| 2.7.1.78 | analysis |
fluorometric method for the determination of the activity of T4 polynucleotide kinase phosphatase PNKP. A partially doublestranded DNA substrate is dephosphorylated on addition of T4 PNKP to generate the long dsDNA, which acts as a template for synthesizing copper nanoclusters. The assay has an analytical range from 0.07 U/ml to 15 U/ml and a detection limit of 0.06 U/ml |
751515 |
| 2.7.1.78 | analysis |
label-free fluorescent assay for T4 polynucleotidekinase/phosphatase PNKP activity using poly(thymine)-templated fluorescent copper nanoparticles as a fluorescent indicator. A T-rich hairpin primer with a 3'-phosphoryl end, serves as both the substrate for T4 PNKP and DNA template for the formation of fluorescent copper nanoparticles. Upon hydrolysis by T4 PNKP, the resulting hairpin primer with a 3'-hydroxyl end is elongated to form a long double-strand product by DNA polymerase, which prohibits the formation of fluorescent copper nanoparticles |
752288 |
| 2.7.1.78 | biotechnology |
application in DNA and RNA sequencing |
642011 |
| 2.7.1.78 | medicine |
A12B4C3 enhances the radiosensitivity of human A549 lung carcinoma and MDA-MB-231 breast adenocarcinoma cells by a factor of two |
703029 |
| 2.7.1.78 | medicine |
human Machado-Joseph disease patients' brain samples show a significant accumulation of DNA strand breaks. PNKP stably associates with ataxin-3, a polyglutamine repeat-containing protein mutated in spinocerebellar ataxia type 3, i.e. Machado-Joseph disease |
751906 |
| 2.7.1.78 | synthesis |
construction of a catalytically useful ribozyme |
642024 |
| 2.7.1.78 | synthesis |
due to reversibility of the reaction, the bacteriophage can be utilized for exchange of labeled phosphate groups between 2 substrates |
642004, 642015 |
| 2.7.1.78 | synthesis |
enzyme is an important tool in the synthesis of genes corresponding to yeast alanine tRNAand to precursor tyrosine tRNA of Escherichia coli |
642011 |
