EC Number   |
Application |
Reference |
|---|
   2.3.2.13 | agriculture |
physiological role of MsTGase and the potential impact of its regulation on MsTGase-associated pest management |
759529 |
| 2.3.2.13 | analysis |
specific tests for measuring the activities of TGase 1 and factor XIII based on their ability to incorporate biotinylated peptides onto spermine-conjugated wells. The rapid and sensitive colorimetric assay shows high sensitivity when TGase isozymes are assayed using their identified preferred substrate peptide. The limit of detection for factor XIII and TGase 1 is as low as 0.01 mU/ml. In each case, good linearity is obtained |
701672 |
| 2.3.2.13 | biotechnology |
after fermentation in presence of enzyme, wheat dough has higher resistance to stretching and lower extensibility than control, dough contains more of the smallest and less large air bubbles. Enzyme improves formation of protein network in bread baked from normal or organic flour but at higher dosage causes uneven ditribution |
658956 |
| 2.3.2.13 | biotechnology |
developments in mTGase engineering together with its role in biomedical applications including biomaterial fabrication for tissue engineering and biotherapeutics, overview |
758686 |
| 2.3.2.13 | biotechnology |
enzyme TGZ treatment effectively improves the textural properties of milk protein concentrate (MPC) gel at strength level and water-holding capacity. Optimal texture of MPC gel is achieved after TGZ treatment using 2 U/g TGZ for 2 h at 35°C and pH 7.0, method evaluation and optimization, overview |
759675 |
| 2.3.2.13 | biotechnology |
in production of homogeneous antibody-drug conjugates, the enzyme is useful for site-specific conjugation of glutamine-based acyl donor substrates and drugs to native and engineered lysines in human immunoglobulins by microbial transglutaminase, overview |
758870 |
| 2.3.2.13 | biotechnology |
microbial transglutaminase (mTG) is used as a crosslinking agent in the preparation of gelatin sponges. The physical properties of the materials are evaluated by measuring their material porosity, water absorption, and elastic modulus. The stability of the sponges are assessed via hydrolysis and enzymolysis, overview. To evaluate the cell compatibility of them TG crosslinked gelatin sponges (mTG sponges), adipose-derived stromal stem cells are cultured and inoculated into the scaffold. Cell proliferation and viability are measured using alamarBlue assay and LIVE/DEAD fluorescence staining, respectively. Cell adhesion on the sponges is observed by scanning electron microscopy. mTG sponges have uniform pore size, high porosity and water absorption, and good mechanical properties. In subcutaneous implantation (in Sprague-Dawley rats), the material is partially degraded in the first month and completely absorbed in the third month. Cell experiments show evident cell proliferation and high viability. The cells grow vigorously and adhered tightly to the sponge. In conclusion, mTG sponge has good biocompatibility and can be used in tissue engineering and regenerative medicine |
759902 |
| 2.3.2.13 | biotechnology |
reaction product of putrescine-pectin conjugate and soy flour protein may be used foredible films with low water vapor permeability and improved mechanical properties |
701653 |
| 2.3.2.13 | biotechnology |
the microbial transglutaminase is used for biotechnological and biomedical engineering, protein engineering by post-translational modification towards the generation of multifunctional conjugates. Biotechnological applications, detailed overview. Transglutaminase-mediated surface immobilization, a widely-used technique to increase stability of labile and cost-intensive enzymes and enable their reuse |
758871 |
| 2.3.2.13 | biotechnology |
use of MTG to site-specifically and covalently immobilize a substrate peptide-tagged protein, e.g. BirA, to a support, i.e. amine-modified magnetic microspheres (MMS) |
758633 |
