Application | Comment | Organism |
---|---|---|
biotechnology | use of MTG to site-specifically and covalently immobilize a substrate peptide-tagged protein, e.g. BirA, to a support, i.e. amine-modified magnetic microspheres (MMS) | Streptomyces mobaraensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development and evaluation of a method for production of a reusable immobilized recombinant His-tagged Escherichia coli biotin ligase (BirA) onto amine-modified magnetic microspheres (MMS) via covalent cross-linking catalyzed using microbial transglutaminase (MTG). The site-specifically immobilized BirA exhibited approximately 95% of enzymatic activity of the free BirA, and without a significant loss in intrinsic activity after 10 rounds of recycling. Method, overview | Streptomyces mobaraensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | P81453 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
microbial transglutaminase | - |
Streptomyces mobaraensis |
MTG | - |
Streptomyces mobaraensis |