EC Number   |
Application |
Reference |
|---|
    1.4.1.1 | analysis |
alanine dehydrogenase from Streptomyces anulatus can be used as a bioreceptor in an ammonium biosensor that can detect ammonium ions in water samples. The sensor shows linear response in the range of 0.1-300mM NH4+ with the detection limit of 0.01 mM NH4+ and response time of 20 s. The sensor is showing good response at wide pH range (pH 5-11) and temperature range (20-50°C) suggesting its usage at ambient and non-ambient conditions. The sensor is successfully validated with Nessler's reagent method by using different water samples |
-, 763656 |
| 1.4.1.1 | analysis |
an enzymatic assay system to eliminate or measure D-Ala, which is reported to affect the taste of seafoods or sake, is constructed using alanine racemase (L-AlaR from Synechocystis sp. PCC6803) and L-alanine dehydrogenase (L-AlaDH from Phormidium lapideum). Using the assay system, the D-Ala contents of 7 crustaceans, i. e. mosa shrimp (Argis lar, Mosaebi), spiny lebbeid (Lebbeus groenlandicus, Oniebi), deepwater prawn (Pandalus eous,Amaebi), flathead lobster (Ibacus ciliates, Uchiwaebi), snow crab (Chionoecetes opilio, Zuwaigani), red snow crab (Chionoecetes japonicus, Benizuwaigani), and horsehair crab (Erimacrus isenbeckii, Kegani), are determined |
762828 |
| 1.4.1.1 | analysis |
determination of L-Ala |
349585 |
| 1.4.1.1 | biotechnology |
heterologous expression of the Bacillus subtilis AlaDH in Lactococcus lactis using the promoter of lactate dehydrogenase from Streptococcus thermophilus leads to a better alanine production in the recombinant strain |
705546 |
| 1.4.1.1 | medicine |
an Ald-specific inhibitor and respiration inhibitory antitubercular drugs, such as Q203 and BDQ, are likely to enable more efficient therapies for tuberculosis |
-, 763354 |
| 1.4.1.1 | medicine |
L-alanine catabolism can be conferred on Mycobacterium bovis BCG by introduction of the gene encoding Ald of Mycobacterium tuberculosis, restoration of Ald activity does not change the in vivo growth of BCG in macrophages or mice, and protection against aerosol Mycobacterium tuberculosis infection is not altered by addition of the gene encoding Ald to the BCG vaccine |
675815 |
| 1.4.1.1 | medicine |
plausible application of Ald inhibitors for treatment of tuberculosis. An Ald-specific inhibitor and respiration inhibitory antitubercular drugs, such as Q203 and BDQ, are likely to enable more efficient therapies for tuberculosis |
-, 763354 |
| 1.4.1.1 | more |
alanine dehydrogenase activity is necessary for an adequate cellular response to nitrogen starvation, is required for efficient degradation of phycobilisomes only during nitrogen starvation |
674289 |
| 1.4.1.1 | nutrition |
Lactococcus lactis strain NZ9000 expressing the enzyme from Bacillus subtilis can be used in imporvement of dairy fermentation for developing healthy yogurts with sweet taste or other fermented dairy foods |
-, 705545 |
| 1.4.1.1 | synthesis |
alterating of cofactor specificity from NADH to NADPH via protein engineering for application as a regenerating enzyme in coupled reactions with NADPH-dependent alcohol dehydrogenases. Mutant D196A/L197R can be applied in a coupled oxidation/transamination reaction of the dicyclic dialcohol isosorbide to its diamines, catalyzed by Ralstonia sp. alcohol dehydrogenase and Paracoccus denitrificans omega-aminotransferase, allowing recycling of NADP+ and L-Ala. Recycling factors of 33 for NADP+ and 13 for L-Ala are observed |
742134 |
