Application | Comment | Organism |
---|---|---|
medicine | L-alanine catabolism can be conferred on Mycobacterium bovis BCG by introduction of the gene encoding Ald of Mycobacterium tuberculosis, restoration of Ald activity does not change the in vivo growth of BCG in macrophages or mice, and protection against aerosol Mycobacterium tuberculosis infection is not altered by addition of the gene encoding Ald to the BCG vaccine | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
into vector pJEX58, resulting plasmid pGS3 expressed in Mycobacterium bovis BCG Pasteur | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
immunoblotting, detectable in sonicates of Mycobacterium bovis BCG harbouring the pGS3 vector | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
no activity in Mycobacterium bovis BCG | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + NAD+ | Mycobacterium tuberculosis displays less Ald activity than the complemented Mycobacterium bovis BCG strain | Mycobacterium tuberculosis | pyruvate + NH3 + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALD | - |
Mycobacterium tuberculosis |
L-alanine dehydrogenase | - |
Mycobacterium tuberculosis |
NAD+ dependent amino acid dehydrogenase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycobacterium tuberculosis |