EC Number |
Application |
Reference |
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1.1.3.17 | agriculture |
expression of CodA in potato plastid genome results in much higher mRNA level of CodA in leaves than in tubers. Glycine betaine accumulates in similar levels in both leaves and tubers of CodA-transplastomic potato plants. The glycine betaine content is moderately increased in transgenic plants, and compartmentation of glycine betaine in plastids confers considerably higher tolerance to drought stress compared to wild-type plants, with higher levels of relative water content and chlorophyll content under drought stress. Transplastomic plants present a significantly higher photosynthetic performance as well as antioxidant enzyme activities during drought stress |
763601 |
1.1.3.17 | agriculture |
introducing of the codA gene into a cereal crop allows the biosynthesis of glycinebetaine by transgenic plants without any need for an exogenous supply of choline or glycinebetaine aldehyde |
389724 |
1.1.3.17 | agriculture |
transformation enables the plants to accumulate glycinebetaine in chloroplasts and significantly enhances the freezing tolerance of plants |
389727 |
1.1.3.17 | analysis |
determination of lead ions by inhibition of choline oxidase enzyme using an amperometric choline biosensor. Choline oxidase is immobilized on a glassy carbon electrode modified with multiwalled carbon nanotubes through cross-linking with glutaraldehyde. In the presence of choline oxidase, choline is enzymatically oxidized into betaine at -0.3 V versus Ag/AgCl reference electrode, lead ion inhibition of enzyme activity causing a decrease in the choline oxidation current. Under the best conditions for measurement of the lowest concentrations of lead ions, the choline oxidase/multiwalled carbon nanotubes/glassy carbon electrode gives a linear response from 0.1 to 1.0 nM Pb2+ and a detection limit of 0.04 nM |
762962 |
1.1.3.17 | analysis |
development of a metal composite material based on zirconium dioxide decorated gold nanoparticles (ZrO2 at AuNPs), copper (I) oxide at manganese (IV) oxide (Cu2O at MnO2) and immobilized choline oxidase (ChOx) onto a glassy carbon electrode (GCE) (ChOx/Cu2O at MnO2-ZrO2 at AuNPs/GCE) for enhancing the electro-catalytic property, sensitivity and stability of the amperometric choline biosensor. The ChOx/Cu2O at MnO2-ZrO2 at AuNPs/GCE displays a good electrocatalytic response to the oxidation of the byproduct H2O2 from the choline catalyzed reaction. The modified electrode also provides a wide linear range of choline concentration from 0.5 to 1000.0 microM with good sensitivity and low detection limit (0.3 microM). The apparent Michaelis-Menten constant is 0.08 mM with Imax of 0.67 microA. The choline biosensor presents high repeatability, good reproducibility, long time of use and good selectivity without interfering effects from possible electroactive species such as ascorbic acid, aspirin, amoxicillin, caffeine, dopamine, glucose, sucrose and uric acid |
762965 |
1.1.3.17 | analysis |
facile and sensitive colorimetric biosensor based on DNAzyme-choline oxidase coupling used for the determination of choline. In this method, choline oxidase produces H2O2 and betaine in the presence of choline and oxygen, then, the DNAzyme converts colorless ABTS into green ABTS+ radicals. The linear range and the limit of detection of this biosensor are 0.1-25 microM and 22 nM. Choline measurement using this biosensor shows satisfactory selectivity and repeatability. Its recovery is 96.95-107.73% in biological samples |
763138 |
1.1.3.17 | analysis |
investigation of an acetylcholinesterase/choline oxidase-based amperometric biosensor as a liqid chromatography detector for acetylcholine determination in brain tissue |
389728 |
1.1.3.17 | analysis |
the immobilized enzyme is used in amperometric biosensors for choline detection, method evaluation |
677170 |
1.1.3.17 | analysis |
two-enzyme sensor for determination of choline esters prepared by covalent co-immobilization of choline oxidase and butyrylcholinesterase |
389725 |
1.1.3.17 | biotechnology |
development and application of enzyme-based gas sensor |
688879 |