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Literature summary for 1.1.3.17 extracted from

  • Magar, H.; Ghica, M.; Abbas, M.; Brett, C.
    Highly sensitive choline oxidase enzyme inhibition biosensor for lead ions based on multiwalled carbon nanotube modified glassy carbon electrodes (2017), Electroanalysis, 29, 1741-1748 .
No PubMed abstract available

Application

Application Comment Organism
analysis determination of lead ions by inhibition of choline oxidase enzyme using an amperometric choline biosensor. Choline oxidase is immobilized on a glassy carbon electrode modified with multiwalled carbon nanotubes through cross-linking with glutaraldehyde. In the presence of choline oxidase, choline is enzymatically oxidized into betaine at -0.3 V versus Ag/AgCl reference electrode, lead ion inhibition of enzyme activity causing a decrease in the choline oxidation current. Under the best conditions for measurement of the lowest concentrations of lead ions, the choline oxidase/multiwalled carbon nanotubes/glassy carbon electrode gives a linear response from 0.1 to 1.0 nM Pb2+ and a detection limit of 0.04 nM Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp. P16101
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Alcaligenes sp.
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