EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.8.1.12 | -999 |
- |
more |
kinetic mechanism |
660445 |
| 1.8.1.12 | -999 |
- |
more |
Km dependent on assay conditions |
394788 |
| 1.8.1.12 | -999 |
- |
more |
Km of several trypanothione disulfide derivatives, e.g. N1-monoglutathionylspermidine, mixed disulfide of N1-monoglutathionylspermidine and glutathione |
394787 |
| 1.8.1.12 | 0.00077 |
- |
NADPH |
in 40 mM HEPES, pH 7.4, 1 mM EDTA |
700087 |
| 1.8.1.12 | 0.0012 |
- |
NADPH |
- |
394792 |
| 1.8.1.12 | 0.0015 |
- |
trypanothione disulfide |
apparent value, in the presence of 0.05 mM DTNB and 0.15 mM NADPH, in 40 mM HEPES, 1 mM EDTA, 0.01% (v/v) BSA, and 0.05% (v/v) Tween 20, pH 7.5, at 22°C |
710883 |
| 1.8.1.12 | 0.0015 |
0.002 |
NADPH |
mutant E201D and E201Q |
394792 |
| 1.8.1.12 | 0.0023 |
0.0066 |
beta-thio-NADPH |
mutant E201D and E201Q |
394792 |
| 1.8.1.12 | 0.003 |
- |
3-acetylpyridine adenine dinucleotide |
mutant E201D and E201Q |
394792 |
| 1.8.1.12 | 0.0039 |
- |
3-acetylpyridine adenine dinucleotide |
- |
394792 |
