EC Number |
General Stability |
Reference |
---|
1.1.1.2 | following incubation at 5°C, pH 8.0 (100 mM Tris/HCl buffer), for 24 h with 30% DMSO and 30% methanol the immobilized enzyme retains 55 and 60%, respectively. Upon increasing the incubation time to 72 h the immobilized enzyme retains 55% and 45% activity, respectively |
728289 |
1.1.1.2 | high concentration of salt, essential to maintain the enzymatic activity and structural integrity of the halophilic enzyme under standard conditions may be partially replaced by DMSO and MeOH |
722209 |
1.1.1.2 | in 3 M KCl the immobilized enzyme retains 80% of its original activity after 9 days at 5°C whereas the free crude enzyme retains 60% under same conditions |
728289 |
1.1.1.2 | presence of NADPH or NADP+ prevents loss of activity |
286168 |
1.1.1.2 | presence of p-mercuribenzoate at pH 7, no loss of activity |
286167 |
1.1.1.2 | stable in the batch reactor |
668054 |
1.1.1.2 | the immobilization process (48 h of incubation of the enzyme with Ni-epoxy Sepabeads support in 100 mM Tris-HCl buffer, pH 8, containing 3 M KCl at 5°C) increases the enzyme stability, thermal activity, and organic solvents tolerance when compared to its soluble counterpart, indicating that the immobilization enhances the structural and conformational stability. |
728289 |
1.1.1.2 | tolerance to high salt concentrations, up to 4 M |
721762 |
1.1.1.2 | unstable |
286210 |