General Stability | Organism |
---|---|
following incubation at 5°C, pH 8.0 (100 mM Tris/HCl buffer), for 24 h with 30% DMSO and 30% methanol the immobilized enzyme retains 55 and 60%, respectively. Upon increasing the incubation time to 72 h the immobilized enzyme retains 55% and 45% activity, respectively | Haloferax volcanii |
in 3 M KCl the immobilized enzyme retains 80% of its original activity after 9 days at 5°C whereas the free crude enzyme retains 60% under same conditions | Haloferax volcanii |
the immobilization process (48 h of incubation of the enzyme with Ni-epoxy Sepabeads support in 100 mM Tris-HCl buffer, pH 8, containing 3 M KCl at 5°C) increases the enzyme stability, thermal activity, and organic solvents tolerance when compared to its soluble counterpart, indicating that the immobilization enhances the structural and conformational stability. | Haloferax volcanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | both soluble and immobilized enzymes display an optimum activity at 4 M KCl, and no activity is detected at zero salt concentration | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
one step purification-immobilization on metal chelate-epoxy Sepabeads | Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NADP+ | - |
Haloferax volcanii | acetaldehyde + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HvADH2 | - |
Haloferax volcanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Haloferax volcanii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
at 60°C the immobilized enzyme shows 20% higher activity compared to the soluble form | Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
assay at | Haloferax volcanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Haloferax volcanii |