EC Number   |
General Stability |
Reference |
|---|
    1.1.1.184 | activation by arachidonic acid leads to a decrease in thermal stability |
347757 |
| 1.1.1.184 | an ammonium sulfate precipitate (3 M, pH 7) of the enzyme loses its activity completely within a few days |
724899 |
| 1.1.1.184 | compared to activity in acetate and Tris-HCl, carbonyl reductase shows higher activity using potassium phosphate as buffer at pH 5.5 or pH 7 |
711492 |
| 1.1.1.184 | glycerol, 20% v/v, stabilizes |
347750 |
| 1.1.1.184 | minor loss of activity in 50 mM phosphate buffer is observed during purification and storage at 4°C without additional precautions |
724899 |
| 1.1.1.184 | no conditions to stabilize the enzyme by freezing at -20°C are found. Addition of 1-10% bovine serum albumin, 20-50% glycerol, 20% 1,2-ethanediol or 0.1% Triton X-100 to the enzyme solution in 40 mM phosphate or 40 mM Tris-HC1 pH 7 before freezing for 1 day or longer has no stabilizing effect |
724899 |
