General Stability | Organism |
---|---|
glycerol, 20% v/v, stabilizes | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Cavia porcellus | |
FeSO4 | - |
Cavia porcellus | |
N-ethylmaleimide | - |
Cavia porcellus | |
NAD+ | - |
Cavia porcellus | |
NADP+ | - |
Cavia porcellus | |
nicotinic acid | weak | Cavia porcellus | |
p-hydroxymercuribenzoate | - |
Cavia porcellus | |
phenylpyruvic acid | - |
Cavia porcellus | |
pyrazole | - |
Cavia porcellus | |
quercetin | - |
Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Cavia porcellus | |
0.002 | - |
pyridine 4-aldehyde | cofactor NADPH | Cavia porcellus | |
0.004 | - |
4-Nitroacetophenone | cofactor NADPH | Cavia porcellus | |
0.009 | - |
menadione | cofactor NADPH | Cavia porcellus | |
0.012 | - |
methyl-1,4-benzoquinone | cofactor NADPH | Cavia porcellus | |
0.015 | - |
pyridine 3-aldehyde | cofactor NADPH | Cavia porcellus | |
0.031 | - |
Benzalacetone | cofactor NADPH | Cavia porcellus | |
0.039 | - |
acetaldehyde | cofactor NADPH | Cavia porcellus | |
0.057 | - |
methyl-1,4-benzoquinone | cofactor NADH | Cavia porcellus | |
0.091 | - |
acetone | cofactor NADPH | Cavia porcellus | |
0.12 | - |
crotonaldehyde | cofactor NADPH | Cavia porcellus | |
0.13 | - |
pyridine 3-aldehyde | cosubstrate NADH | Cavia porcellus | |
0.24 | - |
4-benzoylpyridine | cofactor NADPH | Cavia porcellus | |
0.33 | - |
Benzalacetone | cofactor NADH | Cavia porcellus | |
0.5 | - |
Pyridine-4-aldehyde | cofactor NADH | Cavia porcellus | |
3.1 | - |
acetone | cofactor NADH | Cavia porcellus | |
3.3 | - |
4-benzoylpyridine | cofactor NADH | Cavia porcellus | |
5.1 | - |
acetaldehyde | cofactor NADH | Cavia porcellus | |
250 | - |
crotonaldehyde | cofactor NADH | Cavia porcellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, SDS-PAGE | Cavia porcellus |
86000 | - |
gel filtration | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2 enzyme species | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cavia porcellus |
Storage Stability | Organism |
---|---|
-20°C or 4°C, 20% v/v, glycerol, stable for 1 month | Cavia porcellus |
4°C, 0.02 mg/ml protein concentration, 0.15 M KCl, stable for at least several days, 90% loss of activity after 2 weeks | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-benzoylpyridine + NADPH | - |
Cavia porcellus | ? | - |
? | |
4-nitroacetophenone + NADPH | - |
Cavia porcellus | ? | - |
? | |
acetaldehyde + NADPH + H+ | - |
Cavia porcellus | ethanol + NADP+ | - |
? | |
acetone + NADPH | - |
Cavia porcellus | propan-2-ol + NADP+ | - |
? | |
benzalacetone + NADPH | - |
Cavia porcellus | ? | - |
? | |
crotonaldehyde + NADPH | - |
Cavia porcellus | 2-buten-1-ol + NADP+ | - |
? | |
menadione + NADPH | - |
Cavia porcellus | ? | - |
? | |
methyl-1,4-benzoquinone + NADPH | - |
Cavia porcellus | ? | - |
? | |
additional information | overview | Cavia porcellus | ? | - |
? | |
additional information | NADH also acts as cofactor | Cavia porcellus | ? | - |
? | |
additional information | low reverse direction only detected with cyclohexanol | Cavia porcellus | ? | - |
? | |
pyridine-3-aldehyde + NADPH | - |
Cavia porcellus | ? | - |
? | |
pyridine-4-aldehyde + NADPH | - |
Cavia porcellus | ? | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Cavia porcellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
pH 6-11, 15 min, stable | Cavia porcellus |
50 | - |
pH 6-11, 15 min, about 20% loss of activity | Cavia porcellus |
60 | - |
rapid inactivation above | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
NADPH + 4-nitroacetophenone | Cavia porcellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.4 | - |
unstable | Cavia porcellus |
6 | 11 | 25°C, 15 min, stable | Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Cavia porcellus | |
NADPH | - |
Cavia porcellus |