KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
carnitine | liver homogenate, pH 8.0, temperature not specified in the publication | Mus musculus | |
0.011 | - |
carnitine | mitochondria from liver, pH 8.0, temperature not specified in the publication | Mus musculus | |
0.0328 | - |
CoA | mitochondria from heart, pH 8.0, temperature not specified in the publication | Mus musculus | |
0.0537 | - |
carnitine | heart homogenate, pH 8.0, temperature not specified in the publication | Mus musculus | |
0.0562 | - |
carnitine | mitochondria from heart, pH 8.0, temperature not specified in the publication | Mus musculus | |
0.0664 | - |
CoA | heart homogenate, pH 8.0, temperature not specified in the publication | Mus musculus | |
0.0888 | - |
CoA | mitochondria from liver, pH 8.0, temperature not specified in the publication | Mus musculus | |
0.1134 | - |
CoA | liver homogenate, pH 8.0, temperature not specified in the publication | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P47934 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + carnitine | - |
Mus musculus | CoA + O-acetylcarnitine | - |
r | |
CoA + O-acetylcarnitine | - |
Mus musculus | acetyl-CoA + carnitine | - |
r |
General Information | Comment | Organism |
---|---|---|
physiological function | in the murine cardiomyocyte cytosol, reverse CrAT activity (producing acetyl-CoA) is higher compared with the liver. The heart displays a lower reverse CrAT Km for CoA compared with the liver. Cytosolic reverse CrAT accounts for 4.6% of total activity in heart tissue and 12.7% in H9C2 cells, while highly purified heart cytosolic fractions show significant CrAT protein levels. Acetyl-CoA carboxylase ACC2-knockout mouse hearts show decreased CrAT protein levels and activity, associated with increased palmitate oxidation and acetyl-CoA/CoA ratio compared with controls. Feeding mice a high-fat diet for 10 weeks increases cardiac CrAT protein levels and activity, associated with a reduced acetyl-CoA/CoA ratio and glucose oxidation | Mus musculus |