Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Leishmania infantum |
General Stability | Organism |
---|---|
the enzyme retains 76.02% and 41.17% activity in the presence of 2 M urea after 1 and 24 h, respectively. The enzyme regains activity up to 54.91% after denaturation in 8 M urea. The enzyme is active even after 24 h of guanidine hydrochloride treatment and contains 74.67% and 13.33% activity in 2 M after 1 and 24 h, respectively. Once denatured with 6 M guanidine hydrochloride, the protein regains 45.73% once guanidine hydrochloride is dialyzed | Leishmania infantum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione disulfide + NADPH + H+ | Leishmania infantum | - |
trypanothione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania infantum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography | Leishmania infantum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione disulfide + NADPH + H+ | - |
Leishmania infantum | trypanothione + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trypanothione reductase | - |
Leishmania infantum |
TryR | - |
Leishmania infantum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Leishmania infantum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | less than 15% activity at pH 6.0, about 50% activity at pH 6.5, about 80% activity at pH 7.0, 100% activity at pH 7.5, about 35% activity at pH 8.0, around 30% activity between pH 8.5 and 11.0, no activity at pH 12.0 and less than pH 5.0 | Leishmania infantum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Leishmania infantum | |
NADPH | - |
Leishmania infantum |