Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Leishmania donovani |
General Stability | Organism |
---|---|
the loss of activity at lower denaturant (urea) concentrations is not coincided by dimer dissociation or structural unfolding | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | 1 mM urea has no significant effect on activity whereas 50% enzyme inhibition is observed at 750 mM, enzyme after treatment with urea below 500 mM regains about 95% activity | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Leishmania donovani |
Renatured (Comment) | Organism |
---|---|
8 M urea treated enzyme can also be reactivated up to 50% of native activity | Leishmania donovani |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione disulfide + NADPH + H+ | - |
Leishmania donovani | trypanothione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
trypanothione reductase | - |
Leishmania donovani |
TryR | - |
Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Leishmania donovani | |
NADPH | - |
Leishmania donovani |