Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is highly dependent on ionic strength, optimum activity at 40 mM NaCl | Leishmania donovani | |
additional information | activity is dependent on a redox-active cysteine at the active centre | Leishmania donovani |
Application | Comment | Organism |
---|---|---|
medicine | target for rational approach to design new anti-leishmanial chemotherapeutic agents | Leishmania donovani |
Cloned (Comment) | Organism |
---|---|
cloned into expression vector and expressed in wild-type cells | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antimony chloride | assay containing 10% vol. methanol | Leishmania donovani | |
melarsen-trypanothione disulfide adduct | i.e. adduct of p-(4,6-diamino-S-triazinyl-2-yl)aminophenyl arsenoxide with N1,N8-bis-(glutathionyl)spermidine | Leishmania donovani | |
melarsen-trypanothione disulfide-adduct | - |
Leishmania donovani | |
NaCl | below 5% remaining activity at 1 M | Leishmania donovani | |
potassium antimony tartrate | assay containing 10% vol. methanol | Leishmania donovani | |
triostam | assay containing 10% vol. methanol | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
NADPH | - |
Leishmania donovani | |
0.036 | - |
trypanothione disulfide | - |
Leishmania donovani | |
0.115 | - |
glutathionylspermidine disulfide | - |
Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50900 | - |
2 * 50900, SDS-PAGE | Leishmania donovani |
106000 | - |
gel filtration | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant overexpression of the enzyme from plasmid | Leishmania donovani |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
crude extract of wild-type cells | Leishmania donovani |
2.1 | - |
crude extract cells overexpressing the recombinant enzyme | Leishmania donovani |
112.7 | - |
purified enzyme | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathionylspermidine disulfide + NADPH | - |
Leishmania donovani | 2-glutathionylspermidine + NADP+ | - |
? | |
trypanothione disulfide + NADPH | trypanothione is the oxidized form of N1,N8-bis-(glutathionyl)spermidine, the latter form a stable adduct with melarsen oxide | Leishmania donovani | trypanothione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50900, SDS-PAGE | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
TR | - |
Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
179 | - |
trypanothione disulfide | - |
Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
additional 2 minor optima at pH 6.6 and pH 8.2 | Leishmania donovani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Leishmania donovani | |
NADPH | - |
Leishmania donovani |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
melarsen-trypanothione disulfide-adduct | - |
Leishmania donovani |