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2.7.1.90: diphosphate-fructose-6-phosphate 1-phosphotransferase

This is an abbreviated version!
For detailed information about diphosphate-fructose-6-phosphate 1-phosphotransferase, go to the full flat file.

Word Map on EC 2.7.1.90

Reaction

diphosphate
+
D-fructose 6-phosphate
=
phosphate
+
D-fructose 1,6-bisphosphate

Synonyms

6-phosphofructokinase (pyrophosphate), AtPFPalpha2, diphosphate-D-fructose-6-phosphate 1-phosphotransferase, FBPase, fructose 1,6-bisphosphatase, inorganic pyrophosphate-dependent phosphofructokinase, inorganic pyrophosphate-phosphofructokinase, PFKA, PFP, PfpA4, phosphotransferase, pyrophosphate-D-fructose 6-phosphate 1-, PPi-dependent 6-phosphofructokinase, PPi-dependent phosphofructokinase, PPi-PFK, pyrophosphate D-fructose-6-phosphate 1-phosphotransferase, pyrophosphate-D-fructose 6-phosphate 1-phosphotransferase, pyrophosphate-D-fructose 6-phosphate phosphotransferase, pyrophosphate-dependent 6-phosphofructokinase, pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, pyrophosphate-dependent phosphofructo-1-kinase, pyrophosphate-dependent phosphofructokinase, pyrophosphate-fructose-6-phosphate-phosphotransferase, pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase, pyrophosphate: fructose 6-phosphate 1-phosphotransferase, pyrophosphate: fructose-6-phosphate 1-phosphotransferase, pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase, pyrophosphate:fructose 6-phosphate 1-phosphotransferase, pyrophosphate:fructose-6-phosphate 1-phosphotransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.90 diphosphate-fructose-6-phosphate 1-phosphotransferase

Purification

Purification on EC 2.7.1.90 - diphosphate-fructose-6-phosphate 1-phosphotransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1066fold, to near homogeneity
-
1124fold to near homogeneity
-
167fold
-
2 different methods, method 1: 292fold to homogeneity
-
231fold, to homogeneity
-
40fold, to homogeneity
-
74fold, to homogeneity
-
852fold, to apparent homogeneity
-
approximately 700fold
-
inactivation by gel filtration, reactivation by AMP
-
more than 700fold
-
native, partial 146fold, further purification leads to inactivation
Ni-NTA agarose column chromatography
-
Ni2+NTA column chromatography, gel filtration
Ni2-NTA column chromatography
-
over 500fold
-
over 650fold, to homogeneity
-
partial
partial 83.4fold
-
partial, 102fold
-
partial, 127fold
-
partial, 430fold
-
partial, 569fold
-
partial, approximately 10fold
-
purification to homogeneity from a commercial lyophilized product
-
purification to homogeneity of a commercial lyophilized product
-
recombinant from Escherichia coli, 32fold to homogeneity
recombinant His-tagged protein from Escherichia coli
recombinant protein
-
recombinant wild-type and mutant
-
Sephadex G-25 gel filtration
-
to apparent homogeneity
-
to homogeneity
-
to near homogeneity
-