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2.7.1.21: thymidine kinase

This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.

Word Map on EC 2.7.1.21

Reaction

ATP
+
thymidine
=
ADP
 +
dTMP

Synonyms

2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.21 thymidine kinase

Purification

Purification on EC 2.7.1.21 - thymidine kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
18000fold, 2 different forms
-
3 variants TK-A, TK-B, TK-C
-
3'-aminothymidine-Sepharose resin column chromatography and HisTrap column chromatography
-
DEAE-Sephadex gel filtration
-
Escherichia coli infected with bacteriophage
Tequatrovirus T4
-
expressed in Escherichia coli
Herpes simplex virus
-
expressed in Escherichia coli, homogeneity
-
glutathione-Sepharose column chromatography
-
GST affinity column chromatography
HeLa TK- cells infected with virus
Herpes simplex virus
-
homogeneity
-
Ni-NTA agarose column chromatography
-
Ni-NTA column chromatography
Ni-NTA column chromatography and HiTrap Q column chromatography
-
partial
recombinant enzyme as maltose binding fusion protein from Escherichia coli
recombinant enzyme partially utilizing calmodulin-agarose-beads
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography
-
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli by GST affinity chromatography, cleaving of the tag
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain B834(DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) are immobilized on nickel beads and purified
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged, thrombin-cleavable full length or truncated enzyme from Escherichia coli strain BL21 C41 (DE3) by nickel affinity chromatography, cleavage by thrombin
Herpes simplex virus type 4
-
recombinantly expressed as a His-tag fusion enzyme in Escherichia coli
-
rLyTK1Val-106 and rLyTK1Met-106
wild type and a truncated form missing 243 N-terminal amino acids
-
wild-type and 3 mutants, homogeneity
Herpes simplex virus
-
wild-type and A167Y mutant
Herpes simplex virus
-