2.7.1.144: tagatose-6-phosphate kinase
This is an abbreviated version!
For detailed information about tagatose-6-phosphate kinase, go to the full flat file.
Word Map on EC 2.7.1.144
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2.7.1.144
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aureus
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lactose
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aldolase
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galactose-6-phosphate
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lactococcus
- 2.7.1.144
- aureus
- lactose
- aldolase
- galactose-6-phosphate
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lactococcus
Reaction
Synonyms
D-tagatose 6-phosphate kinase, D-tagatose-6-phosphate kinase, Lacc, phosphotagatokinase, tagatose 6-phosphate kinase (phosphorylating)
ECTree
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Crystallization
Crystallization on EC 2.7.1.144 - tagatose-6-phosphate kinase
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high resolution structures of D-tagatose-6-phosphate kinase (LacC) in two crystal forms. The structures define LacC in apoform, in binary complexes with ADP or the cofactor analogue adenosine 5'-(beta,gamma-imino)triphosphate, and in a ternary complex with adenosine 5'-(beta,gamma-imino)triphosphate and D-tagatose-6-phosphate. LacC adopts a closed structure required for phosphoryl transfer only when both substrate and co-factor are bound
native and L124M, L125M double mutant crystallized by hanging drop vapor diffusion method in apoform, in binary complexes with ADP or the cofactor analogue AMP-PNP, and in a ternary complex with AMP-PNP and D-tagatose-6-phosphate, wild-type protein give crystals that diffracte only to low resolution, the SeMet double mutant protein produced much more ordered crystals