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2.5.1.85: all-trans-nonaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific]

This is an abbreviated version!
For detailed information about all-trans-nonaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific], go to the full flat file.

Word Map on EC 2.5.1.85

Reaction

geranylgeranyl diphosphate
+ 5 isopentenyl diphosphate = 5 diphosphate +
all-trans-nonaprenyl diphosphate

Synonyms

At-SPS1, At-SPS2, AtSPS1, AtSPS2, EC 2.5.1.11, HbSDS, long-chain prenyl diphosphate synthase, long-chain solanesyl diphosphate synthase, SlSPS, solanesyl diphosphate synthase, solanesyl-diphosphate synthase, solanesyl-diphosphate synthase 1, SPPS, SPS1, Sps2, TbSPPS, TCSPPS

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.85 all-trans-nonaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific]

Cloned

Cloned on EC 2.5.1.85 - all-trans-nonaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific]

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
expression in Escherichia coli, His6-tagged At-SPS2 fusion protein and the truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)
expression of TcSPPS is able to complement an Escherichia coli ispB mutant
gene AtSPS1, overexpressing of the plastoquinone-9 biosynthesis gene SPS1 in Arabidopsis thaliana generating plants that specifically accumulate plastoquinone-9 and its derivative plastochromanol-8
gene AtSPS1, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Nicotiana benthamiana epidermis cell endoplasmic reticulum using Agrobacterium tumefaciens strain C58C1 for transfection
gene AtSPS2, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Nicotiana benthamiana leaf chloroplasts using Agrobacterium tumefaciens strain C58C1 for transfection
gene SlSPS, constitutive overexpression of SlSPS in immature tobacco leaves elevates the plastoquinone content in the leaves
-
gene SlSPS, recombinant expression in Escherichia coli. In planta, constitutive overexpression of SlSPS elevates the plastoquinone content of immature tobacco leaves
-
heterologous expression of either SPS1 allows the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli
heterologous expression of SPS1 allows the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli
heterologously expressed in Escherichia coli as as His6-tagged fusion protein