2.5.1.23: sym-norspermidine synthase
This is an abbreviated version!
For detailed information about sym-norspermidine synthase, go to the full flat file.
Reaction
Synonyms
HbSpe 1, propylaminc transferase
ECTree
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Temperature Stability
Temperature Stability on EC 2.5.1.23 - sym-norspermidine synthase
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120
-
no protection by substrate against thermal inactivation at 120°C is observed by incubating the enzyme in the presence of polyamines
25 - 85
-
protein retains its quarternary structure between 25°C and 28°C. A highly reversible subtle conformational transition is detected by numerous structure-dependent techniques over 40-45°C, with a midpoint centered at 45°C
87
-
the mechanism by which the protein achieves thermal stabilization is driven by a conformational equilibrium between two forms of different stability. The stability of each form towards denaturation is characterized by a specific temperature dependence. The low temperature form (form A), is stable over 12-89°C. Its stability maximum is 36.8 kJ/mol at 50°C. Form B, which is populated at higher temperature, spans the interval 28-146°C. Its stability maximum is 71.6 kJ/mol at 87°C
90
-
rapidly looses activity above 90°C and can no longer be considered native