2.4.1.292: GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase
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For detailed information about GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase, go to the full flat file.
Reaction
3 UDP-N-acetyl-alpha-D-galactosamine + = 3 UDP +
Synonyms
PglH, pglH2
ECTree
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Engineering
Engineering on EC 2.4.1.292 - GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase
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E267A
site-directed mutagenesis, the mutant shows an about 300fold decrease in the turnover rate compared to the wild type enzyme
E275A
site-directed mutagenesis, the mutant shows an about 300fold decrease in the turnover rate compared to the wild type enzyme
H118A
site-directed mutagenesis, the mutant exhibits a reduced turnover rate compared to wild-type
K68A
site-directed mutagenesis, the mutant exhibits a reduced turnover rate compared to wild-type
K75A
site-directed mutagenesis, the mutant exhibits a reduced turnover rate compared to wild-type
K75A, R72A
site-directed mutagenesis, the mutant exhibits a reduced turnover rate compared to wild-type
K75A/R72A/K68A
site-directed mutagenesis, the mutant exhibits a reduced turnover rate compared to wild-type
R191A
R72A
site-directed mutagenesis, the mutant exhibits a reduced turnover rate compared to wild-type
R72A/K68A
site-directed mutagenesis, the mutant exhibits a reduced turnover rate compared to wild-type
T271A
site-directed mutagenesis, the mutant exhibits a fivefold reduction in turnover rate compared to wild-type
H371R
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site-directed mutagenesis, strain FA 1090, the mutation is sufficient to restore PglH activity in the wild-type FA1090 strain
additional information
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construction of pglH::kanr mutants, and complementation studies. The pglH::kanr mutant of strain 81116 has reduced colonization efficiency in a chick model. Mutation of pglH in strains 11168H and 81116 results in the reduced attachment to and invasion of Caco-2 cells. Reduction in attachment and invasion for strain 81116 are 23fold and 15fold, respectively, whereas the corresponding figures for strain 11168H are 6fold and and 5.4fold, respectively
additional information
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construction of pglH::kanr mutants, and complementation studies. The pglH::kanr mutant of strain 81116 has reduced colonization efficiency in a chick model. Mutation of pglH in strains 11168H and 81116 results in the reduced attachment to and invasion of Caco-2 cells. Reduction in attachment and invasion for strain 81116 are 23fold and 15fold, respectively, whereas the corresponding figures for strain 11168H are 6fold and and 5.4fold, respectively
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additional information
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replacement of the strain N400 locus carrying the deleted form of ORFs 2 and 3 with those derived from the Neisseria gonorrhoeae strain FA1090 and from diverse neisserial strains into Neisseria gonorrhoeae strain N400 in which the pgl gene function and associated glycan structures is defined, mutant glycosylation patterns, overview. The presence of a stereotypic, conserved deletion mutation is inactivating pglH in strains of Neisseria gonorrhoeae, Neisseria meningitidis, and related commensals
additional information
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replacement of the strain N400 locus carrying the deleted form of ORFs 2 and 3 with those derived from the Neisseria gonorrhoeae strain FA1090. The presence of a stereotypic, conserved deletion mutation is inactivating pglH in strains of Neisseria gonorrhoeae, Neisseria meningitidis, and related commensals