gene CGI_10023521, DNA and amino acid sequence determination and analysis, the Pacific oyster encodes three isozymes of glycogenin: CgGN-alpha, CgGN-beta, and CgGN-gamma, genetic structure and sequence comparisons, phylogenetic tree. The isolated three CgGN isoforms contain alternative exon regions. PCR-based quantitative expression analysis. Recombinant expression of FLAG-tagged enzymes in HEK-293T cells, recombinant expression of EGFP-tagged enzyme from pEGFP-N1-CgGN in HeLa cells
gene GYG1, recombinant expression of functional GST-tagged human muscle glycogen synthase in complex with human glycogenin-1 in Spodoptera frugiperda Sf9 cells using a bicistronic pFastBac-Dual expression vector and baculovirus transfection
preparation of a truncated already glucosylated His-tag fusion protein in Escherichia coli ER2566, expression of the truncated non-glucosylated His-tag fusion protein in Escherichia coli CGSC 4997
recombinant expression of His6-tagged wild-type and mutant enzymes glycogenin-1 in HEK-293 cells. Coexpression of wild-type glycogenin-2 together with different glycogenin-1 variants using a cell-free system, coexpression of functional glycogenin-1 and glycogenin-2 increases the glucosylation of glycogenin-2
recombinant expression of His6-tagged wild-type and mutant glycogenin-2 in HEK-293 cells. Coexpression of wild-type glycogenin-2 together with different glycogenin-1 variants using a cell-free system, coexpression of functional glycogenin-1 and glycogenin-2 increases the glucosylation of glycogenin-2