2.4.1.182: lipid-A-disaccharide synthase
This is an abbreviated version!
For detailed information about lipid-A-disaccharide synthase, go to the full flat file.
Word Map on EC 2.4.1.182
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2.4.1.182
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acyltransferase
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raetz
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clockwise
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udp-glcnac
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lipopolysaccharide
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paralogue
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lps
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udp-2,3-diacylglucosamine
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castellanii
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cotranscribed
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amoebae
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micelle
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pneumophila
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bacteriol
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acanthamoeba
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glycerophospholipids
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resin
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udp-n-acetylglucosamine
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synthesis
- 2.4.1.182
- acyltransferase
-
raetz
-
clockwise
- udp-glcnac
- lipopolysaccharide
- paralogue
- lps
- udp-2,3-diacylglucosamine
- castellanii
-
cotranscribed
-
amoebae
-
micelle
- pneumophila
-
bacteriol
-
acanthamoeba
- glycerophospholipids
- resin
- udp-n-acetylglucosamine
- synthesis
Reaction
Synonyms
lcsC, lcsC/lpxB1, Legionella cytotoxic suppressor C, lipid A disaccharide synthase, lipid A disaccharide synthetase, LpxB, More, pgsB, synthase, lipid A disaccharide
ECTree
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Cloned
Cloned on EC 2.4.1.182 - lipid-A-disaccharide synthase
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expressed in Escherichia coli MN7 (temperature sensitive lpxB mutant), expression of protein complements growth at 30 and 42°C
expression from plasmid, DNA sequence analysis, lpxB gene is cotranscribed with the lpxA gene located in the same operon
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gene lpxB, recombinant expression of His6-tagged wild-type and mutant enzymes, coexpression of His6-tagged mutants LpxBR201A, LpxBN316A, and LpxBFN, and pull-down assays
gene lpxB, recombinant overexpression of LpxB in Escherichia coli resulting in the accumulation of aberrant tubular membranes of a uniform diameter along the inner surface of the bacterial inner membrane, suggesting that the accumulation of the lipid product of the LpxB reaction, 2',3'-diacylglucosamine-(beta,1'-6)-2,3-diacylglucosamine-1-phosphate (DSMP), is toxic to cells
overexpression in Escherichia coli from plasmid, alone and together with gene lpxA, UDP-GlcNAc acyltransferase
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