2.4.1.128: scopoletin glucosyltransferase

This is an abbreviated version!
For detailed information about scopoletin glucosyltransferase, go to the full flat file.

Reaction

Synonyms

71C1, 71C3, glucosyltransferase, uridine diphosphoglucose-scopoletin, N-feruloyltyramine glucosyltransferase, NbUGT73A24, NbUGT73A25, scopoletin UGT, SGTase, TOGT, UDP-glucose:phenylpropanoid glucosyltransferase, UDP-glucose:scopoletin glucosyltransferase, UDPglucose:scopoletin O-beta-D-glucosyltransferase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.128 scopoletin glucosyltransferase

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Results	in table
1	Activating Compound
3	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
6	General Information
3	Inhibitors
24	kcat/KM [mM/s]
26	KM Value [mM]
1	Localization
2	Metals/Ions
3	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
9	Organism
3	Pathway
3	pH Optimum
3	Protein Variants
3	Purification (Commentary)
1	Reaction
1	Reaction Type
8	Reference
8	Source Tissue
5	Specific Activity [micromol/min/mg]
20	Substrates and Products (Substrate)
1	Subunits
12	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
16	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 2.4.1.128 - scopoletin glucosyltransferase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.76 - 0.92

carvacrol

2 entries

31.5 - 155

kaempferol

2 entries

0.035 - 0.09

N-4-trans-coumaroyl tyramine

2 entries

0.4 - 0.44

N-caffeoyl-O-methyl tyramine

2 entries

0.02 - 0.041

N-trans-feruloyl tyramine

2 entries

61.4 - 185.71

quercetin

2 entries

0.84 - 210.7

scopoletin

7 entries

0.21 - 13.05

UDP-glucose

5 entries

0.76

carvacrol

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

0.92

carvacrol

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

31.5

kaempferol

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

155

kaempferol

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

0.035

N-4-trans-coumaroyl tyramine

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

0.09

N-4-trans-coumaroyl tyramine

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

0.4

N-caffeoyl-O-methyl tyramine

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

0.44

N-caffeoyl-O-methyl tyramine

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

0.02

N-trans-feruloyl tyramine

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

0.041

N-trans-feruloyl tyramine

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

61.4

quercetin

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

185.71

quercetin

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

0.84

scopoletin

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

1.21

scopoletin

Arabidopsis thaliana

O82381, Q9LML7

wild type, 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C

702478

2.82

scopoletin

Nicotiana benthamiana

-

recombinant enzyme, pH 7.5, 30°C

759956

28.48

scopoletin

Arabidopsis thaliana

O82381, Q9LML7

71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476)

702478

28.48

scopoletin

Arabidopsis thaliana

O82381, Q9LML7

71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C

702478

166.6

scopoletin

Arabidopsis thaliana

O82381, Q9LML7

71C1 double mutant

702478

210.7

scopoletin

Arabidopsis thaliana

O82381, Q9LML7

wild type

702478

0.21

UDP-glucose

Arabidopsis thaliana

O82381, Q9LML7

wild type, 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C

702478

3.92

UDP-glucose

Arabidopsis thaliana

O82381, Q9LML7

71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476)

702478

3.92

UDP-glucose

Arabidopsis thaliana

O82381, Q9LML7

71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C

702478

9.45

UDP-glucose

Arabidopsis thaliana

O82381, Q9LML7

71C1 double mutant

702478

13.05

UDP-glucose

Arabidopsis thaliana

O82381, Q9LML7

wild type

702478