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D137A
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the mutant shows slightly reduced activity compared to the wild-type enzyme
G138A
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the mutant shows slightly reduced activity compared to the wild-type enzyme
G355I
mutation improves the rate of reaction
H132L
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the replacement of His132 leads to reduced enzyme activity
H132L/H133L
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inactive mutant
H133L
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the replacement of His133 leads to highly reduced enzyme activity
A360F
Marinobacter nauticus
mutation slightly improves the rate of reaction found for smaller fatty alcohols
A360I
Marinobacter nauticus
mutation improves the rate of reaction found for smaller fatty alcohols
A360V
Marinobacter nauticus
mutation improves the rate of reaction found for smaller fatty alcohols
L356A
Marinobacter nauticus
mutation increases selectivity toward ethanol
L356F
Marinobacter nauticus
mutation increases selectivity toward ethanol, with a drop in specific activity of approximately 89% for dodecanol
L356V
Marinobacter nauticus
mutation increases selectivity toward 2-phenylethanol
L356Y
Marinobacter nauticus
substantial loss of activity
M405A
Marinobacter nauticus
significant decrease in overall activity with dodecanol
M405F
Marinobacter nauticus
mutation increases selectivity toward isoamyl alcohol, significant decrease in overall activity with dodecanol
M405L
Marinobacter nauticus
significant decrease in overall activity with dodecanol
M405W
Marinobacter nauticus
dramatically shifts selectivity toward ethanol, significant decrease in overall activity with dodecanol
A360F
Marinobacter nauticus DSM 11845
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mutation slightly improves the rate of reaction found for smaller fatty alcohols
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A360I
Marinobacter nauticus DSM 11845
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mutation improves the rate of reaction found for smaller fatty alcohols
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A360V
Marinobacter nauticus DSM 11845
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mutation improves the rate of reaction found for smaller fatty alcohols
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L356A
Marinobacter nauticus DSM 11845
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mutation increases selectivity toward ethanol
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L356F
Marinobacter nauticus DSM 11845
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mutation increases selectivity toward ethanol, with a drop in specific activity of approximately 89% for dodecanol
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L356V
Marinobacter nauticus DSM 11845
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mutation increases selectivity toward 2-phenylethanol
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L356Y
Marinobacter nauticus DSM 11845
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substantial loss of activity
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M405F
Marinobacter nauticus DSM 11845
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mutation increases selectivity toward isoamyl alcohol, significant decrease in overall activity with dodecanol
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N36R
mutant enzyme is capable of synthesizing very long chain fatty acyl-harboring wax esters
additional information
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construction of knockout mutants by transposon mutagenesis
additional information
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establishment of heterologous wax ester biosynthesis in a recombinant Escherichia coli strain by coexpression of a fatty alcohol-producing bifunctional acyl-coenzyme A reductase from the jojoba plant, Simmondsia chinensis, and a bacterial wax ester synthase from Acinetobacter baylyi strain ADP1, catalyzing the esterification of fatty alcohols and coenzyme A thioesters of fatty acids, succesful production of jojoba oil-like wax esters, such as palmityl oleate, palmityl palmitoleate, and oleyl oleate, in presence of oleate, but also of fatty acid butyl esters, overview
additional information
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the knockout mutant Acinetobacter sp. strain ADP1acr1omegaKm is unable to produce fatty alcohols
additional information
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the knockout mutant Acinetobacter sp. strain ADP1acr1omegaKm is unable to produce fatty alcohols
-
additional information
co-expression of fatty acyl-CoA reductase and wax synthase in Saccharomyces cerevisiae results in synthesis of myristyl myristate, myristyl palmitoleate, myristyl palmitate and palmityl myristate when fed with myristic acid
additional information
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co-expression of fatty acyl-CoA reductase and wax synthase in Saccharomyces cerevisiae results in synthesis of myristyl myristate, myristyl palmitoleate, myristyl palmitate and palmityl myristate when fed with myristic acid
additional information
generation of single EgWSD2 and double knockout EgWSD2/5 mutant cells, phenotypes, overview
additional information
generation of single EgWSD2 and double knockout EgWSD2/5 mutant cells, phenotypes, overview
additional information
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generation of single EgWSD2 and double knockout EgWSD2/5 mutant cells, phenotypes, overview
additional information
generation of single EgWSD5 and double knockout EgWSD2/5 mutant cells, phenotypes, overview
additional information
generation of single EgWSD5 and double knockout EgWSD2/5 mutant cells, phenotypes, overview
additional information
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generation of single EgWSD5 and double knockout EgWSD2/5 mutant cells, phenotypes, overview
additional information
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the wax diester synthase activity of DGAT1 helps to explain the deficiency of type II wax diesters in the fur lipids of Dgat-/- mice
additional information
domain swap experiment between the mouse acyl-CoA:wax alcohol acyltransferase (AWAT2) and the homologous mouse acyl-CoA:diacylglycerol O-acyltransferase 2 (DGAT2). Te substrate specificity of AWAT2 is partially determined by two predicted transmembrane domains near the amino terminus of AWAT2. Upon exchange of the two domains for the respective part of DGAT2, the resulting chimeric enzyme is capable of incorporating up to 20% of very long acyl chains in the wax esters upon expression in Saccharomyces cerevisiae. The amount of very long acyl chains in wax esters synthesized by wild type AWAT2 is negligible. The effect is due to a single amino acid exchange within one of the predicted membrane domains, the AWAT2 N36R
additional information
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domain swap experiment between the mouse acyl-CoA:wax alcohol acyltransferase (AWAT2) and the homologous mouse acyl-CoA:diacylglycerol O-acyltransferase 2 (DGAT2). Te substrate specificity of AWAT2 is partially determined by two predicted transmembrane domains near the amino terminus of AWAT2. Upon exchange of the two domains for the respective part of DGAT2, the resulting chimeric enzyme is capable of incorporating up to 20% of very long acyl chains in the wax esters upon expression in Saccharomyces cerevisiae. The amount of very long acyl chains in wax esters synthesized by wild type AWAT2 is negligible. The effect is due to a single amino acid exchange within one of the predicted membrane domains, the AWAT2 N36R
additional information
cloned cDNA is expressed in transgenic Arabidopsis thaliana plants, in combination with cDNAs encoding the jojoba fatty acyl-CoA reductase and a beta-ketoacyl-CoA synthase from Lunaria annua under a seed specific promotor, highly enhanced wax content in transgenic seeds
additional information
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cloned cDNA is expressed in transgenic Arabidopsis thaliana plants, in combination with cDNAs encoding the jojoba fatty acyl-CoA reductase and a beta-ketoacyl-CoA synthase from Lunaria annua under a seed specific promotor, highly enhanced wax content in transgenic seeds
additional information
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generation of an atfG25OMEGAApr insertion mutant of Streptomyces sp. G25