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2.1.1.282: tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase

This is an abbreviated version!
For detailed information about tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase, go to the full flat file.

Reaction

S-adenosyl-L-methionine
+
7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
=
S-adenosyl-L-homocysteine
 +
7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe

Synonyms

aTrm5a methyltransferase, aTrm5a-type enzyme, aTrm5a/Taw22-like enzyme, More, NEQ228, PAB2272, PaTrm5a, SSO2439, Taw21, TAW22, Trm5a, Trm5a/Taw22-like enzyme, tRNAPhe:imG2 methyltransferase, TYW3, YGL050w

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.282 tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase

Engineering

Engineering on EC 2.1.1.282 - tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C301S/C308S/C326S
site-directed mutagenesis
D243A
site-directed mutagenesis, substrate binding compared to wild-type enzyme
E173A
site-directed mutagenesis, the mutant shows 9 and 26% of wild-type activity for imG and imG2 formation, respectively
E213A
F165A
site-directed mutagenesis, inactive mutant
H128A
site-directed mutagenesis, substrate binding compared to wild-type enzyme
P260N
site-directed mutagenesis, the mutant shows no and 114% of wild-type activity for imG and imG2 formation, respectively
P262A
site-directed mutagenesis, the mutant shows 5 and 8% of wild-type activity for imG and imG2 formation, respectively
R133A
site-directed mutagenesis, substrate binding compared to wild-type enzyme
R134A
site-directed mutagenesis, the mutant shows 2 and 4% of wild-type activity for imG and imG2 formation, respectively
R135A
site-directed mutagenesis, substrate binding compared to wild-type enzyme
R174A
V21C/C301S/C308S/K314C/C326S
site-directed mutagenesis
Y318A
site-directed mutagenesis, substrate binding compared to wild-type enzyme