2.1.1.140: (S)-coclaurine-N-methyltransferase
This is an abbreviated version!
For detailed information about (S)-coclaurine-N-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.140
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2.1.1.140
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benzylisoquinoline
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n-methylated
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japonica
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coptis
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isoquinoline
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norreticuline
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s-reticuline
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n-methyltransferases
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norcoclaurine
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poppy
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scoulerine
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somniferum
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3\'-hydroxylase
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4\'omt
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magnoflorine
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6-o-methyltransferase
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opium
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papaver
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tetrahydroisoquinoline
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aporphine
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medicine
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biotechnology
- 2.1.1.140
-
benzylisoquinoline
-
n-methylated
- japonica
- coptis
- isoquinoline
- norreticuline
-
s-reticuline
- n-methyltransferases
- norcoclaurine
- poppy
- scoulerine
- somniferum
-
3\'-hydroxylase
-
4\'omt
- magnoflorine
-
6-o-methyltransferase
-
opium
-
papaver
- tetrahydroisoquinoline
-
aporphine
- medicine
- biotechnology
Reaction
Synonyms
(S)-Coclaurine N-methyltransferase, 6OMT, CjCNMT, CMT, CNMT, CNMT protein, coclaurine N-methyltransferase, coclaurine-N-methyltransferase, FspC, S-adenosyl-L-methionine:coclaurine N-methyltransferase
ECTree
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Application
Application on EC 2.1.1.140 - (S)-coclaurine-N-methyltransferase
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biotechnology
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CNMT should be quite useful for biotransformation of the intermediates of alkaloid biosynthesis to N-methyltransferred products
medicine
a Saccharomyces cerevisiae strain is engineered to express seven heterologous enzymes (Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR)), resulting in protoberberine alkaloid production from a simple benzylisoquinoline alkaloid precursor. A number of strategies are implemented to improve flux through the pathway, including enzyme variant screening, genetic copy number variation, and culture optimization. This leads to an over 70-fold increase in canadine titer up to 1.8 mg/l. Increased canadine titers enable extension of the pathway to produce berberine, a major constituent of several traditional medicines in a microbial host. This strain is viable at pilot scale