1.2.1.87: propanal dehydrogenase (CoA-propanoylating)
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For detailed information about propanal dehydrogenase (CoA-propanoylating), go to the full flat file.
Reaction
Synonyms
BphJ, CoA-acylating aldehyde dehydrogenase, coenzyme A-acylating aldehyde dehydrogenase, More, PduP, TTHB247
ECTree
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Engineering
Engineering on EC 1.2.1.87 - propanal dehydrogenase (CoA-propanoylating)
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I195F
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created by site-specific mutagenesis, no significant reduction of channeling efficiency of the enzyme complex toward acetaldehyde or propionaldehyde
additional information
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design of a coenzyme A (CoA) dependent n-butanol biosynthesis pathway tailored to the metabolic physiology of the cyanobacterium Synechococcus elongatus PCC 7942 by incorporating an ATP driving force and a kinetically irreversible trap. Oxygen-sensitive CoA-acylating butyraldehyde dehydrogenase (Bldh) is exchanged for the oxygen-tolerant PduP from Salmonella enterica. Replacing Bldh with PduP in the n-butanol synthesis pathway results in n-butanol production to a cumulative titer of 404 mg/l with peak productivity of 51 mg/l per day, exceeding the base strain by 20fold. Anaerobic growth rescue of Escherichia coli strain JCL166 by overexpression of the Clostridium butanol pathway with different aldehyde dehydrogenases PduP