1.14.13.69: alkene monooxygenase
This is an abbreviated version!
For detailed information about alkene monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.69
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1.14.13.69
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epoxidation
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xanthobacter
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vinyl
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diiron
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nocardioides
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4-monooxygenase
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propyne
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dinuclear
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rhodochrous
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smmos
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epoxygenation
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epoxyethane
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corallinus
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autotrophicus
- 1.14.13.69
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epoxidation
- xanthobacter
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vinyl
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diiron
- nocardioides
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4-monooxygenase
- propyne
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dinuclear
- rhodochrous
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smmos
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epoxygenation
- epoxyethane
- corallinus
- autotrophicus
Reaction
Synonyms
AkMO, alkene epoxygenase, alkene monooxygenase, AMO, ethene MO, EtnABCD, EtnC, etnD, IsoMO, isoprene monooxygenase, More, PmoABCD, propene MO, propene monooxygenase, XAMO
ECTree
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Subunits
Subunits on EC 1.14.13.69 - alkene monooxygenase
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heterotetramer
hexamer
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alpha2beta2gamma2, 2 * 58100, 2 * 38000, 2 * 9650, calculated from deduced amino acid sequences, masses in SDS-PAGE appear to be smaller (alpha and gamma subunits) or larger (beta subunit)
additional information
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1 * 36000, subunit EtnA, + 1 * 12000 subunit EtnB, + 1 * 59000, subunit EtnC, + 1 * 37000, subunit EtnD, SDS-PAGE
heterotetramer
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1 * 39000, subunit PmoA, + 1 * 12000 subunit PmoB, + 1 * 57000, subunit PmoC, + 1 * 37000, subunit PmoD, SDS-PAGE
heterotetramer
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1 * 36000, subunit EtnA, + 1 * 12000 subunit EtnB, + 1 * 59000, subunit EtnC, + 1 * 37000, subunit EtnD, SDS-PAGE
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heterotetramer
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1 * 39000, subunit PmoA, + 1 * 12000 subunit PmoB, + 1 * 57000, subunit PmoC, + 1 * 37000, subunit PmoD, SDS-PAGE
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additional information
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the alkene MO consists of a small hydroxylase subunit EtnA of 36 kDa, a coupling protein subunit EtnB of12 kDa, a large hydroxylase subunit EtnC of 59 kDa, and a reductase subunit EtnD of 37 kDa
additional information
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the alkene MO consists of a small hydroxylase subunit PmoA of 39 kDa, a coupling protein subunit PmoB of12 kDa, a large hydroxylase subunit PmoC of 57 kDa, and a reductase subunit PmoD of 37 kDa
additional information
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the alkene MO consists of a small hydroxylase subunit EtnA of 36 kDa, a coupling protein subunit EtnB of12 kDa, a large hydroxylase subunit EtnC of 59 kDa, and a reductase subunit EtnD of 37 kDa
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additional information
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the alkene MO consists of a small hydroxylase subunit PmoA of 39 kDa, a coupling protein subunit PmoB of12 kDa, a large hydroxylase subunit PmoC of 57 kDa, and a reductase subunit PmoD of 37 kDa
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additional information
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the four component IsoMO is likely to comprise an oxygenase (IsoABE), reductase (IsoF), coupling protein (IsoD) and Rieske-type ferredoxin (IsoC). The alpha subunit of the oxygenase component of SDIMOs (equivalent to IsoA in IsoMO) contains an active site diiron center required for catalytic activity of the enzyme. The oxygenase component might form dimers in an (alphabetagamma)2 arrangement. The beta subunit (IsoE) and the gamma subunit (IsoB) combine with the alpha subunit to form the oxygenase complex
additional information
the enzyme contains NADH-oxidoreductase and a Rieske-type ferredoxin components and the binuclear non-haem iron active site, it requires a small catalytic coupling/effector protein, AamD
additional information
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enzyme contains 4 components: 1. a monomeric 35500 Da NADH reductase containing 1 mol of FAD and a probable 2Fe-2S center, mass spectrometry 2. a dimeric ferredoxin consisting of two 13300 Da subunits, each containing a Rieske-type 2Fe-2S cluster, SDS-PAGE 3. a 11000 Da monomeric protein that contains no detectable cofactors, mass spectrometry 4. a 212000 Da alpha2beta2gamma2 multimeric protein containing 4 atoms of nonheme iron
additional information
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x * 58037, oxygenase alpha-subunit, + x * 9740, gamma-subunit, + x * 13359, ferredoxin, + x * 11193, coupling or effector protein, + 38188, oxygenase beta-subunit, + x * 34171, reductase subunit, calculation from nucleotide sequence
additional information
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x * 58037, oxygenase alpha-subunit, + x * 9740, gamma-subunit, + x * 13359, ferredoxin, + x * 11193, coupling or effector protein, + 38188, oxygenase beta-subunit, + x * 34171, reductase subunit, calculation from nucleotide sequence
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additional information
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enzyme contains 4 components: 1. a monomeric 35500 Da NADH reductase containing 1 mol of FAD and a probable 2Fe-2S center, mass spectrometry 2. a dimeric ferredoxin consisting of two 13300 Da subunits, each containing a Rieske-type 2Fe-2S cluster, SDS-PAGE 3. a 11000 Da monomeric protein that contains no detectable cofactors, mass spectrometry 4. a 212000 Da alpha2beta2gamma2 multimeric protein containing 4 atoms of nonheme iron
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