1.14.13.180: aklavinone 12-hydroxylase
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For detailed information about aklavinone 12-hydroxylase, go to the full flat file.
Reaction
Synonyms
aklavinone 12-hydroxylase, DnrF, RdmE
ECTree
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Application
Application on EC 1.14.13.180 - aklavinone 12-hydroxylase
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synthesis
biosynthesis of carminic acid from glucose in engineered Escherichia coli. Expression of an optimized type II polyketide synthase machinery from Photorhabdus luminescens enables a high-level production of flavokermesic acid upon coexpression of the cyclases ZhuI and ZhuJ from Streptomyces sp. R1128. Mutants of aklavinone 12-hydroxylase (DnrF) from Streptomyces peucetius and C-glucosyltransferase (GtCGT) from Gentiana triflora with 2-5fold enhanced conversion efficiencies can perform hydroxylation and C-glucosylation of flavokermesic acid, respectively, resulting in 0.63 mg/l of carminic acid production from glucose