1.14.11.54: mRNA N1-methyladenine demethylase

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For detailed information about mRNA N1-methyladenine demethylase, go to the full flat file.

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Synonyms

ABH3, AlkB, AlkB homolog 3, ALKBH3, alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, PCA-1, prostate cancer antigen-1

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

                1.14.11.54 mRNA N1-methyladenine demethylase

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Results	in table
11	AA Sequence
4	Application
2	Cloned(Commentary)
1	Crystallization (Commentary)
194	Disease/ Diagnostics
8	General Information
5	kcat/KM [mM/s]
5	KM Value [mM]
4	Localization
1	Metals/Ions
3	Natural Substrates/ Products (Substrates)
13	Organism
5	Protein Variants
1	Purification (Commentary)
1	Reaction
13	Reference
11	Source Tissue
14	Substrates and Products (Substrate)
2	Subunits
15	Synonyms
1	Systematic Name
5	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.14.11.54 - mRNA N1-methyladenine demethylase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

5'-dAAAA-1-methyl-AYYAAA + 2-oxoglutarate + O2

5'-dAAAAAYYAAA + formaldehyde + succinate + CO2

Homo sapiens

Q96Q83

-

742749

-

-

?

N1-methyladenine in mRNA + 2-oxoglutarate + O2

adenine in mRNA + formaldehyde + succinate + CO2

3 entries

N1-methyladenine in ssDNA + 2-oxoglutarate + O2

adenine in ssDNA + formaldehyde + succinate + CO2

Homo sapiens

Q96Q83

-

742749

-

-

?

N1-methyladenine in tRNA + 2-oxoglutarate + O2

adenine in tRNA + formaldehyde + succinate + CO2

Homo sapiens

Q96Q83

-

765483

-

-

?

N3-ethylthymidine in mRNA + 2-oxoglutarate + O2

thymidine in mRNA + ?

Mus musculus

Q8K1E6

-

737316

-

-

?

N3-methylcytidine in mRNA + 2-oxoglutarate + O2

cytidine in mRNA + formaldehyde + succinate + CO2

Bombyx mori

-

-

765803

-

-

?

N3-methylcytidine in tRNA + 2-oxoglutarate + O2

cytidine in tRNA + formaldehyde + succinate + CO2

Homo sapiens

Q96Q83

-

765483

-

-

?

N6-methyladenine in tRNA + 2-oxoglutarate + O2

adenine in tRNA + formaldehyde + succinate + CO2

Bombyx mori

-

-

765803

-

-

?

RNA-N1-methyladenine + 2-oxoglutarate + O2

RNA-adenine + formaldehyde + succinate + CO2

Escherichia coli

P05050

-

741601

-

-

?

RNA-N6-methyladenine + 2-oxoglutarate + O2

RNA-adenine + formaldehyde + succinate + CO2

Escherichia coli

P05050

-

741601

-

-

?

additional information

?

-

Bombyx mori

-

ALKBH3 demethylates 3-meC, 1-meA, and N6-meA in the presence of 2-oxoglutarate and Fe(II). ALKBH3 preferentially demethylates N6-meA in tRNA

765803

-

-

-

N1-methyladenine in mRNA + 2-oxoglutarate + O2

adenine in mRNA + formaldehyde + succinate + CO2

Bombyx mori

-

-

765803

-

-

?

N1-methyladenine in mRNA + 2-oxoglutarate + O2

adenine in mRNA + formaldehyde + succinate + CO2

Homo sapiens

Q96Q83

-

741808, 742749

-

-

?

N1-methyladenine in mRNA + 2-oxoglutarate + O2

adenine in mRNA + formaldehyde + succinate + CO2

Homo sapiens

-

N1-methyladenine is prevalent in Homo sapiens mRNA, which shows an N1-methyladenine/adenine ratio of about 0.02%. Methylation of adenine in mRNA is reversible by ALKBH3. Recombinant ALKBH3 protein has robust demethylation activity against a synthetic RNA substrate containing a sitespecifically incorporated m1A modification

739137

-

-

?